Sequences from confirmation amplicon


Confirmation sequences for line 010F09 and hit At2g23050 are shown.
Line ID 010F09
Locus At2g23050 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:9812455
Primers used for PCR KN42 - 8474, show primer details
   
Sequence Name 85-K103173-0022-010-F09C-8409
GenBank Accession LN514535 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>85-K103173-0022-010-F09C-8409
CATTTACAATTGAATATTCCTGATAGTCATGGTCCCATTCATCATCCTCAGTGTTTAGAG
TAGTAGTGGTAAGCTCAGGAGTGCTATATCCTGTTGATGAACCATTGGCTCTAATCTGTT
CAAAGAAGAGAACTTGCACAATCACCCGCATTGGCAAACGTTCGTTCTGTACCGCATGTT
CACACGCTTCCGCGGATAGTTTCCTACAATCCATTAGCCCGCATACTCTCTTCTTCTCGC
TCTTGCTAATCCCCGAATGTTCCTGAAAATTTTCCAAATTATTAACAAATCAAGAAACGT
TATGAAAAACAGAGGATTTGAATCTTGTTTTACCTTTAGAAACATGTCAATTGCGCGATA
TAACACGTCGTGAGAGTG
   
Sequence Name 86-K103173-0022-010-F09C-KN42
GenBank Accession LN514645 [GenBank]
Sequencing primer gene specific primer, KN42: 5'-ACACTCTCACGACGTGTTATATCG-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K103173-0022-010-F09C-KN42
TAACAGATTCAATCCTCTGTTTTTCATAACGTTTCTTGATTTGTTAATAATTTGGAAAAT
TTTCAGGAACATTCGGGGATTAGCAAGAGCGAGAAGAAGAGAGTATGCGGGCTAATGGAT
TGTAGGAAACTATCCGCGGAAGCGTGTGAACATGCGGTACAGAACGAACGTTTGCCAATG
CGGGTGATTGTGCAAGTTCTCTTCTTTGAACAGATTAGAGCCAATGGTTCATCAACAGGA
TATAGCACTCCTGAGCTTACCACTACTACTCTNAACACTGAGGATGATGAATGGGACCAT
GACTATTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAG
CAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAAT
TCAAAAATGTAGATGTCCGCAGCGTTATNATAAAATGAAAGTACATTTTGATAAAACGAC
AAATTACGATCCGTCGTATTTATNGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAA
TCTTTATNTCGACGTGTCTACNTTCACGTCCAAATGGGGGCTTAGATGAGAAACTTCACG
ATCGATGCCTTG

Last Updated on 06.12.2018 16:11