Sequences from confirmation amplicon


Confirmation sequences for line 010G04 and hit At1g33600 are shown.
Line ID 010G04
Locus At1g33600 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12182027
Primers used for PCR NS37 - 8474, show primer details
   
Sequence Name 88-K102437-0022-010-G04C-8409
GenBank Accession LN514850 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K102437-0022-010-G04C-8409
CCATTTACTCATTGACTAGTCTCTCACTTAAATTCAGCTTCCCCATGTCGAATCGAAGTT
TGTTCCCTGACGCTTGAAACTCATATAGGTTATGCGCTAGGTTGAAGAACCATGTTAGAC
TCCCTGAGATCTCGTTTTCTGACAGATCGATGTAAAAGTAAATATTGGGTCTCACTGGCT
TCCAATTGTCTAAGCTCATATTGATCCCGCATTTTACCAGCTTCAACGAGTACATGGACG
GCGACGAGGTCACCCATTTCGGTATCGTTTTGAGGTGAAATTGGTTGTATGAAAGATCTA
GAGTGGCAAGGCCATCAACGTTCTTCATGGCAGGGAGTGGACCAGTTAGAAAATTGTGGG
AGAGATTGAGAACCNNTNTTTNCTTTTNCATCGATCTGTCAGAAAACGAGATCTCAGGGA
GTCTAACATGGTTCTTCAACCTAGCGCATAACCTATATGAGTTTCAAGCGTCAGGGAACA
AACTTCGATTCGACATGGGGAAGCTGAATTTAAGTGAGAGACTA
   
Sequence Name 88-K102438-0022-010-G04C-NS37
GenBank Accession LN514851 [GenBank]
Sequencing primer gene specific primer, NS37: 5'-TCTCAATCTCTCCCACAATTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>88-K102438-0022-010-G04C-NS37
CGTTGATGGCCTTGCCACTCTAGATCTTTCATACACCAATTTCACCTCAAAACGATACCG
AAATGGGTGACCTCGTCGCCGTCCATGTACTCGTTGAAGCTGGTAAAATGCGGGATCAAT
ATGAGCTTAGACAATTGGAAGCCAGTGAGACCCAATATTTACTTTTACATCGATCTGTCA
GAAAACGAGATCTCAGGGAGTCTAACATGGTTCTTCAACCTAGCGCATAACCTATATGAG
TTTCAAGCGTCAGGGAACAAACTTCGATTCGACATGGGGAAGCTGAATTTAAGTGAGAGA
CTAGTCAATGAGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATTCACGTCCAAATGGG

Last Updated on 06.12.2018 16:11