Sequences from confirmation amplicon


Confirmation sequences for line 010G06 and hit At2g16370 are shown.
Line ID 010G06
Locus At2g16370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:7082084
Primers used for PCR 17mk - 8474, show primer details
   
Sequence Name 26-K114225-0022-010-G06C-17mk
GenBank Accession LN498030 [GenBank]
Sequencing primer gene specific primer, 17mk: 5'-CATTATCCACACCTTTTGTTTGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K114225-0022-010-G06C-17mk
CAGCATATGAATTACAGTTATAGTTGTATACCAATTATAACACCACAACAGTTATAGTAT
ATTAGAAAGATTATTTATGTATCTTGTAGACAAACTTAAAACCAAAAAGAACATAAATTG
GCCAAAATCATCAAAGTTCTCATTCGATAGTTGAAATAGGAAACAATATATAAAAGAAGT
ATAAAATCCTAAATCGCTCTAGAACCATGTGGAACTAGAGGATTCCAACACATGTTTATG
ACAAGTTATCACCCACAAAAGGGTAATATGAGTCCATACATTGATGGTCATTCATCTGAT
TCAATCATAAATTTTACACAATCAATTTGTCGGTAGTCTCCTTTTGGTCATATCCTATAG
TAAGAGATGAGAACTAAACCGCCNTTTTCATTTCTATCTTCTTGTGAGGATCATAGCCTG
CATTACCTGCAGTAGTGCATTTTTATGTCGACTACTGCAGGTAATGAGGAATGCATTTCT
ATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATG
   
Sequence Name 06-K114224-0022-010-G06C-8409
GenBank Accession LN488905 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K114224-0022-010-G06C-8409
TTACATTGAATATAGAAATGCATTCCTCATTACCTGCAGTAGTCGACATAAAAATGCACT
ACTGCAGGTAATGCAGGCTATGATCCTCACAAGAAGATAGAAATGAAAATGGCGGTTTAG
TTCTCATCTCTTACTATAGGATATGACCAAAAGGAGACTACCGACAAATTGATTGTGTAA
AATTTATGATTGAATCAGATGAATGACCATCAATGTATGGACTCATATTACCCTTTTGTG
GGTGATAACTTGTCATAAACATGTGTTGGAATCCTCTAGTTCCACATGGTTCTAGAGCGA
TTTAGGATTTTATACTTCTTTTATATATTGTTTCCTATTTCAACTATCGAATGAGAACTT
TGATGATTTTGGCCAATTTATGTTCTTTTTGGTTTTAAGTTTGTCTACNAGATACATAAA
TAATCTTTCTAATATACTATAACTGTTGTGGTGTTATAATTGGTATACAACTATAACTGT
AATTTCATATGCTTGTGTTTAAAGTAATTGATTGATGACACAAACAAAA

Last Updated on 06.12.2018 16:11