Sequences from confirmation amplicon


Confirmation sequences for line 010H02 and hit MGH6 are shown.
Line ID 010H02
Locus MGH6 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:4116317
Primers used for PCR 01wb - 3144, show primer details
   
Sequence Name 23-K118209-0022-010-H02C-01wb
GenBank Accession LN496745 [GenBank]
Sequencing primer gene specific primer, 01wb: 5'-CCCTACAAAATCAAGATGCCTAAG-3'
Sequence (A. th genome BLAST matches underlined) 
>23-K118209-0022-010-H02C-01wb
TCAAAGTATCCTTTGTTCCCATATTTTATATTAGCATTTTGGCAAGTGAGTGGGAAATTA
GTGCTGATCTAACTTCGTAAGGCAGTGATAAACTTGCAAACTTTATAACATGTATTTTTG
CCTATTTCTTAAAACTCAAACAGTCACATGCCACTTTTCTTGATCTCCACTATGTTGAGG
CGATGACATGTTCTAGTTGAGCTGAAAAGTTTGGTTTAAAGATACATATCTAAGACATGA
ATGAGCACATCTTGAAATCAAACCGATTAAACTGAACTCAACATGAATACTGAAAACAAT
GATTCAATTGCATTGTGGATCAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAAT
CTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGG
TCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAG
   
Sequence Name 11-K118207-0022-010-H02C-3144
GenBank Accession LN491318 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K118207-0022-010-H02C-3144
CCTTTCTCCATATAAGGAAGTTCATTTTCATTTGGAGAGGACAGGGTACCCGGGGATCAG
ATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGATCCACAATGCAATTGAATC
ATTGTTTTCAGTATTCATGTTGAGTTCAGTTTAATCGGTTTGATTTCAAGATGTGCTCAT
TCATGTCTTAGATATGTATCTTTAAACCAAACTTTTCAGCTCAACTAGAACATGTCATCG
CCTCAACATAGTGGAGATCAAGAAAAGTGGCATGTGACTGTTTGAGTTTTAAGAAATAGG
CAAAAATACATGTTATAAAGTTTGCAAGTTTATCACTGCCTTACGAAGTTAGATCAGCAC
TAATTTCCCACTCACTTGCCAAAATGCTAATATAAAATATGGGAACAAAGATACTATTGA
TTGGATTCTGCAGATGAAAAAAAGGTGTCGCAATCATCCAACTTAGGCATC

Last Updated on 06.12.2018 16:11