Sequences from confirmation amplicon


Confirmation sequences for line 011A07 and hit At5g19830 are shown.
Line ID 011A07
Locus At5g19830 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:6703678
Primers used for PCR 01vo - 3144, show primer details
   
Sequence Name 47-K118077-0022-011-A07C-01vo
GenBank Accession LN506329 [GenBank]
Sequencing primer gene specific primer, 01vo: 5'-TGCTACTGCACTTCTTCTGTTCAT-3'
Sequence (A. th genome BLAST matches underlined) 
>47-K118077-0022-011-A07C-01vo
ATACAAAGGAACAGACACAACGTACTCAAATGTTCTTTTTGGTGATAATGCATTTAAAAC
AACTAGGATCTGGTTGTGTTGATTTTCAAATAGTTTCGATGTGTGCTCTTGTTTTGTTGT
GCTGCAGATTGGATTCGAAATGATTGATGTTTTCGCTGAGTCAGTGGGGATTCAAATGAA
CTTGGTAAATTTCAAGGCTATAATGGCTTCCATACATTCAGTTTAAACCGAAGGCGGGAA
ACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAG
AGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATAT
CAATCCAATCCACAACA
   
Sequence Name 22-K118076-0022-011-A07C-35St
GenBank Accession LN496300 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K118076-0022-011-A07C-35St
CTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTGAATGTATGGAAGCCATTATA
GCCTTGAAATTTACCAAGTTCATTTGAATCCCCACTGACTCAGCGAAAACATCAATCATT
TCGAATCCAATCTGCAGCACAACAAAACAAGAGCACACATCGAAACTATTTGAAAATCAA
CACAACCAGATCCTAGTTGTTTTAAATGCATTATCACCAAAAAGAACATTTGAGTACGTT
GTGTCTAGTTCCTTTGTATTTGTCACCTGGATTGCCCAGTCCTAGAAACAGCCATGGCCG
ATGAACAGAAG

Last Updated on 06.12.2018 16:11