Sequences from confirmation amplicon


Confirmation sequences for line 011C04 and hit At4g12290 are shown.
Line ID 011C04
Locus At4g12290 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:7305132
Primers used for PCR BW40 - 8474, show primer details
   
Sequence Name 01-K104337-0022-011-C04C-BW40
GenBank Accession LN486558 [GenBank]
Sequencing primer gene specific primer, BW40: 5'-GATCCATGTACGGAACAAAAAGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K104337-0022-011-C04C-BW40
TCTCATGAGTATCCGGGTCGTGTACTCTTACCCGTGATATTACCACACCTGCTCTCGGGT
CAGGTTTTAGATGAAATTCCCAATTTGCCCATTTCACTAGATGGTTGTCCTCTATCACGA
AGCTTGGACCACGTGGCTGCTCAATGGATATCGGGTTTAGAGGCCGAGTTTTGTCGGTGG
TTGCGAGCTTTTGGAAGCGGTAATCGGTATTGGTTGAACCGGGTATGGGTATAGCCCGAC
CTGTATCGGTTATCTCGATCACTTGCTTTGTATCTAAATCGATGAGAATGGTTAAACCTT
CGATAGGACGCATGTAGAAGTTAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATT
   
Sequence Name 17-K104338-0022-011-C04C-8409
GenBank Accession LN493834 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K104338-0022-011-C04C-8409
TTGAATAATCCTAACTTCTACATGCGTCCTATCGAAGGTTTAACCATTCTCATCGATTTA
GATACAAAGCAAGTGATCGAGATAACCGATACAGGTCGGGCTATACCCATACCCGGTTCA
ACCAATACCGATTACCGCTTCCAAAAGCTCGCAACCACCGACAAAACTCGGCCTCTAAAC
CCGATATCCATTGAGCAGCCACGTGGTCCAAGCTTCGTGATAGAGGACAACCATCTAGTG
AAATGGGCAAATTGGGAATTTCATCTAAAACCTGACCCGAGAGCAGGTGTGGTAATATCA
CGGGTAAGAGTACACGACCCGGATACTCATGAGACACGTGACGTGATGTACAAAGGTTTC
GTGTCTGAACTTTTTGTTCCGTACATGGATCA
Primers used for PCR BW41 - 3144, show primer details
   
Sequence Name 70-K104377-0022-011-C04C-BW41
GenBank Accession LN512384 [GenBank]
Sequencing primer gene specific primer, BW41: 5'-CAACCACGACCTATTCCTAAACAT-3'
Sequence (A. th genome BLAST matches underlined) 
>70-K104377-0022-011-C04C-BW41
AAACCATGATCACGTGTCCGATACACCAAACCATCCTCTAGACCCACTCACGGTGTCGGA
GATTAACAAAATCCGATCAATACTCTCTTCTCACGCGCTATTCACTTCCGGTACACCACA
CGCGCTCCACACCGTCGTTCTTGAAGAGCCTGAGAAGAATCTTGTCCGTCACTGGGAAAA
AGGGAACCCACTTCCTCCGAGGAAAGCTTCCGTCATCGCACGTGTTGGCGCGGACACGCA
CGTGCTCACCGTTGATATCTCTACGGGTCGGGTAGATTCAGAGAATAGCCCGGTTCGTGT
TTCTGGTTACCCGATGATGACTATAGAAGAGATGAACGATATCACTGTTGTACCATTTTC
AAACGCGGATTTCAACCGTACGATCATCTCTCGTGGAGTTAATCTAACGGACGTGATTTG
TTTCCCAATCTCTTGTGGTTGGTTTGGTAATAAAGAAGAAAACGCGAGGGTAATTAAAAG
TCAGTGTCCGAATGGCTTTATGGAGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGA
TCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTT
GCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATTC
   
Sequence Name 86-K104378-0022-011-C04C-3144
GenBank Accession LN514653 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>86-K104378-0022-011-C04C-3144
CCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCTCCATAAAGCCATTCGGA
CACTGACTTTTAATTACCCTCGCGTTTTCTTCTTTATTACCAAACCAACCACAAGAGATT
GGGAAACAAATCACGTCCGTTAGATTAACTCCACGAGAGATGATCGTACGGTTGAAATCC
GCGTTTGAAAATGGTACAACAGTGATATCGTTCATCTCTTCTATAGTCATCATCGGGTAA
CCAGAAACACGAACCGGGCTATTCTCTGAATCTACCCGACCCGTAGAGATATCAACGGTG
AGCACGTGCGTGTCCGCGCCAACACGTGCGATGACGGAAGCTTTCCTCGGAGGAAGTGGG
TTCCCTTTTTCCCAGTGACGGACAAGATTCTTCTCAGGCTCTTCAAGAACGACGGTGTGG
AGCGCGTGTGGTGTACCGGAAGTGAATAGCGCGTGAGAAGAGAGTATTGATCGGATTTTG
TTAATCTCCGACACCGTGAGTGGGTCTAGAGGATGGTTTGGTGTATCGGACACGTGATCA
TGGTTTTTGGTATTTGGTTTAGGATCATGTTTAGG

Last Updated on 06.12.2018 16:11