Sequences from confirmation amplicon


Confirmation sequences for line 011F01 and hit F26P21 are shown.
Line ID 011F01
Locus F26P21 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15944185
Primers used for PCR EV20 - 8474, show primer details
   
Sequence Name 76-K037328-022-011-F01C-EV20
GenBank Accession LN513345 [GenBank]
Sequencing primer gene specific primer, EV20: 5'-GGATCCATTTTCTTTTGTTGTGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K037328-022-011-F01C-EV20
TGTCCCCATGATCATGACGTATTCTTTTTATATTGGTCTCACCTAAAACATTATGTCCTA
AGATTCTATATGTAAGATTCCATTTCCATCCATATTCTTATAAAATACAATAAAACGAAT
CTTTGTCAATGATTCGAATTGCGTTTGAGAAAGATCGTATACTTAGCTCCATTTACACCT
ACCCATAGAGTTCTCCCTAACTGGCTAAATAAATATGCTAGTGTTATTGGTTAAGAGGTT
GTAATTACATATTGTATATGTAGATGCAAGTCTTAAGTCTAGAAATTTACATGTCTTGCT
TTGCTTTGAATTTTAGAAAAGCTTCGGTAGAGGAGATTTCAAACCAGGATATCACACGGG
CATTAATACAGTATCAAATATGATATATACCTTACAATATATAGAGTTGTTTCAAAAAGA
TGATTTTGGAAACACGCTAAAACCCCAAACAATGATCATGATTCAAAAGAAAGATATAAG
GTATATACTCTGCATCAGTTTGAAATGTAAATAAAATTATCATAATGTTATAAGAAACAA
GAAAAATAAAAGAAGAGAAGATGATAATTTAAAAAAAAGAAAGAAAGAGTATGATGGCTT
CAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTC
AATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTC
CGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGT
ATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGT
CTACATTCA
   
Sequence Name 83-K037328-022-011-F01C-8409
GenBank Accession LN514271 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K037328-022-011-F01C-8409
GAGCCATTTCAATTGAAGCCATCATACTCTTTCTTTCTTTTTTTTAAATTATCATCTTCT
CTTCTTTTATTTTTCTTGTTTCTTATAACATTATGATAATTTTATTTACATTTCAAACTG
ATGCAGAGTATATACCTTATATCTTTCTTTTGAATCATGATCATTGTTTGGGGTTTTAGC
GTGTTTCCAAAATCATCTTTTTGAAACAACTCTATATATTGTAAGGTATATATCATATTT
GATACTGTATTAATGCCCGTGTGATATCCTGGTTTGAAATCTCCTCTACCGAAGCTTTTC
TAAAATTCAAAGCAAAGCAAGACATGTAAATTTCTAGACTTAAGACTTGCATCTACATAT
ACAATATGTAATTACAACCTCTTAACCAATAACACTAGCATATTTATTTAGCCAGTTAGG
GAGAACTCTATGGGTAGGTGTAAATGGAGCTAAGTATACGATCTTTCTCAAACGCAATTC
GAATCATTGACAAAGATTCGTTTTATTGTATTTTATAAGAATATGGATGGAAATGGAATC
TTACATATAGAATCTTAGGACATAATGTTTTAGGTGAGACCAATATAAAAAGAATACGTC
ATGATCATGGTGGACATTAAAAAAAGACTCGAAGAGATATCACAACAA

Last Updated on 06.12.2018 16:11