Sequences from confirmation amplicon


Confirmation sequences for line 011F11 and hit At1g69120 are shown.
Line ID 011F11
Locus At1g69120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:25982394
Primers used for PCR 04kb - 8474, show primer details
   
Sequence Name 38-K113916-0022-011-F11C-04kb
GenBank Accession LN503057 [GenBank]
Sequencing primer gene specific primer, 04kb: 5'-ATTTGATTTTCCTTTGTTGTACGG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K113916-0022-011-F11C-04kb
AATTCTCGAAACCGACTCTTGAAACCCGTTTAACAACCGCAACCTTCGGCCGCTTTCGCC
GCATGAAGCATTTCCATATATATATTTCGTAATCGTCAACAATAAAAACAGTTTGCCACA
TACATATAAATAGTGGCTAGGCTCTTTTCATCCAATTAATATATTTTGGCAAATGTTCGA
TGTTCTTATATCATCATATATAAATTAGCAGGCTCCTTTCTTCTTTTGTAATTTGATAAG
TTTATTTGCTTCAATATGGAGCAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAA
ATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTAC
CAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 10-K113915-0022-011-F11C-8409
GenBank Accession LN490743 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K113915-0022-011-F11C-8409
GCAAATAAAACTTCTTCAAATTCCAAAAGAAGAAAGGGGCCCGCTAATTTATATACGAAG
ATATAAGAAACATCGAACATTTGCCAAAATATATTAATCGGATGAAAAGAGCCTAGCCAC
TATTTATATGTATGTGGCAAACTGTTTTTATTGTTGACGATTACAAATATATATATGGAA
ATGCTTCATGCGGCGAAGCAGCCAAGGTTGCAGTTGTAAACGGGTTCAAGAGTCAGTTCG
AGATCATTCCTCCTCATTGCCATAGGATCATCTTCTTGATACAGACCACTGCATTCACAA
TCATACATAGGATATATATGAAAGTGCATATGTAATCATATGTTTCACCGTACAACA

Last Updated on 06.12.2018 16:11