SimpleSearch - Line and FST details
Line specific information
| Line ID | 011F11 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401031) |
| Segregation Analysis | 50:47:45 |
| Confirmed for Hit | At1g69120 At3g14270 |
| Parent of DUPLO pair | 12625 |
| Parent of pair(s) | none |
Gene hit At1g34550
| Sequence (A. th genome BLAST matches underlined) | >86-K012256-022-011-F11-8409 TGGGATCCTCCCTATAGTGAGTCGTATTACTCAAGGNGAAGGAGTGTTTGTTGATAAGTC TTGATGATTATCTAAAGTATGGCATTGCCTCCATGTGTGGAAGCTGGGTTTAGAATAGTA ATGTCAAAAGTGCTCTTGACATGATATGAGCTGAAGTCCTCCTATAAACTATGGGATCCT CCCTATAGTGAGTCGTATTACTCAGNNGGANNANTGAGNGGGGGTTGNTGAATGGGAGGC CTTTTTGTATGAGGGGATTGATTGTTGTTCTTTTTTTGGGGCACTGCACCGGGGGGGGGC CCTTATGCGGGACCGATTATATAGGGGGGGGGTCTTCCCCCGCCCACAGGGGGGGCGAGA GGGGAGAGAACCCCCGGGGGTGGCCTTGAAATCGGCGCTTCCAACCGCGGGGGGGGTTTT TTTCCAGGGGGGTGGGGTCCTCTTTTGGGGGGAAAACACCCGGGGTCCCCAAAGGAAGAT CTGGAGGGGGCCCATTTGGGGGGAGAAAAACAAAAAGGATAATCTCCCCTCCCCTTTTTC ACTTTCAAAAAGGCGGCGGGGGCGCGCGGGGGGTTTTTTTATGAAAGGGAAGGGGCCCTT TGTGTGAAAAAAACACAAAAATAGACAACCGCGCGTTTTTTTTTTGGGGCGAAGCGAATA AACACCATATTTTTTAAATTGGGGGAAAAT |
| GenBank Accession | AL752270 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:12647852 - go to primer design |
| BLAST e Value | 2e-40 |
| Hit Clone Code (BAC ID) | F12K21 |
| Hit Gene Code | At1g34550 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | transmembrane protein (DUF616) |
| Insertion Classification | CDSi |
| Confirmation Status | unknown |
Gene hit At1g69120
| Sequence (A. th genome BLAST matches underlined) | >88-K016930w-022-011-F11T-8409 TGATACTGAGATTTCCCGGACATGAAGCCATTTACTACTCGAATATATCCTGCTCCATAT AGAGGCAATGACACTCATCATATTACAAAAGAAGAACGGAGCCTGCTAATTTATATAGGA TGCCCTCAGAACATCGAGCATTTGCCAGCATATATGAATTGGATGAAAACAGCCTAGGTC ATGATGCCTGTCGACAGAGCCGCATCTCGATAGATCCCTTGCCTCGACTAGGACGCCTAT GATATCGTCCCGATAATGCGATATACTTCTGAAACTTCTTGTACTGCCCTGATCTTGCGT GCTACTGCGCCGCCGAGTGTATCGATACGGACAGGAACGTTGGCTCATACATTGTGATGG ACGGGCTA |
| GenBank Accession | FR800125 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:25982380 - go to primer design |
| BLAST e Value | 1e-22 |
| Hit Clone Code (BAC ID) | F4N2 |
| Hit Gene Code | At1g69120 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | K-box region and MADS-box transcription factor family protein |
| Insertion Classification | TS2TE (3') |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR800125 [GenBank] |
Gene hit At4g27350
| Sequence (A. th genome BLAST matches underlined) | >86-K013045-022-011-F11-35St NACTGACGTAAGGGATGACGCACANTCCCCTATCCTTCGCAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGGGATTGAACTCATAAAGCTCGACTATTGATTCTATGGGATCCT CTCTATCGTGAATCCGATTGCTTCGGGAGGAGGCGAAGAGACGGTCAACAATTTTATCCA ATGTCGGGCCGTCTTTTCGCCTGTTTCGGCCTCAAGAAAGCTTCAACTAAACCTATCTCT GGTGGTAACAATTCCGCCGCCGTAGCCGCAGAAATTCCCGCCGGAGATGGTCCCGTTCTC GTCCAGCTATTCTCGTCTCAGGGATGTAAAACATCGCCCGCGGAGGAGATGTTGATGTCC CGGCTATGGGATCCTC |
| GenBank Accession | AL752271 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr4:13692706 - go to primer design |
| BLAST e Value | 2e-120 |
| Hit Clone Code (BAC ID) | M4I22 |
| Hit Gene Code | At4g27350 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | membrane lipoprotein lipid attachment site-like protein, putative (DUF1223) |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | unknown |
Gene hit At3g14270
| Sequence (A. th genome BLAST matches underlined) | >86-K013043-022-011-F11-35St NATTGACGTAAGGGATGACGCACANTCCCCTATCCTTCGCAGACCCTTCCTCTATATAAG GAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCT TCGGTTTAAACTATCAGTGTTTGAACTCAGGAGAGCTCGACTATTGATTCTATGGGATCC TCCCTATAGTGAGTCGTATTACTCAGGGGTGAGGCCAAGAGACTCGCCA |
| GenBank Accession | FR800122 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr3:4756444 - go to primer design |
| BLAST e Value | 0.38 |
| Hit Clone Code (BAC ID) | MLN21 |
| Hit Gene Code | At3g14270 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | phosphatidylinositol-4-phosphate 5-kinase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | FR800122 [GenBank] |
Gene hit MVA3
| Sequence (A. th genome BLAST matches underlined) | >88-K016981w-022-011-F11T-8409 TGATCTGANATTTCCCGGACATGAAGCCATTTACATTGAATATATCCTGCTCCATATTGA AGCAAATAAACTTATCAAATTACAAAAGAAGAAAGGAGCCTGCTAATTTATATATGATGA TATAAGAACATCGAACATTTGCCAAAATATATTAATTGGATGAAAAGAGCCTATGGGATC TCCCCTATGAGTGAGAGACGACTATGTTTGAAAAGTCGTGATCGGTTGAGATTGCGTTGC AAAGTGGTCTACTCAGCCAAAATACTTCTTAATCACATCAATCGACCTTCAGGTTTGGAG TTTTCTCCATTTTCAATTTTTTCGAATCCTATGGGATCCTCCCCTATAGTGAG |
| GenBank Accession | AL935991 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr5:5905387 - go to primer design |
| BLAST e Value | 2e-70 |
| Hit Clone Code (BAC ID) | MVA3 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At2g06965
| Sequence (A. th genome BLAST matches underlined) | >86-K012433-022-011-F11-8409 TTCCCTGGACATGAAGCCTTTACACTTGAATATATCCAGGCTGCATTTTCTTGCATATTA TGGACTACCAATCAGAATAGACCTTGCTAATAGATTAGAGCGGCCGCATAAAGACATTCT TATTACTGAAGATGCCTATGGGATCCTCCCTATAGTGAGTCGTATTACTCACCATACTTC GTTGCGAGGCCCAAACACTTCACGACGAACAAAAATGTTGGCTAGCCGTCATCAAAAGAA ACTGTTTTCACCATCTGTAAGCCAAAAGTGATCAGGACATGATATGAAAAGAAATACTCG TACAGACGATGGGATACTCCACATATACATATATATGACAGACTTCAGCTGCGACCAAAA ACTCTTGGAATCGATAAGGAGGAGCATATCAATCCATCTCGGGGGAATCGGCTTTAAATG TACAGGCACATTGCAAGCTCTCAGAATTAGTGTCACAACTCCTCCCACAACCATTCTTCC TGGTTTCTTAAGTCCCTTAGCCCATCCTCTGTAACTTAACAAGTGCTTGATTANGTAGAA TGAGACCGAAGCGGTAGTCTGATCTCCAGCTA |
| GenBank Accession | FR800121 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:2875515 - go to primer design |
| BLAST e Value | 2e-101 |
| Hit Clone Code (BAC ID) | T4E14 |
| Hit Gene Code | At2g06965 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | |
| Insertion Classification | TS2TE |
| Confirmation Status | unknown |
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Last Updated on 10.06.2021 13:37 |
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