Sequences from confirmation amplicon


Confirmation sequences for line 011G10 and hit At3g58390 are shown.
Line ID 011G10
Locus At3g58390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21601153
Primers used for PCR MC22 - 3144, show primer details
   
Sequence Name 84-K035351-022-011-G10C-MC22
GenBank Accession LN514385 [GenBank]
Sequencing primer gene specific primer, MC22: 5'-TTCCAAGGAATCGATGTTTTTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>84-K035351-022-011-G10C-MC22
GCCCGGTTCACTTACTTGCTCGTTCTCCATGAAATTCTTCGCGCGTATGCTCATTATATC
ACCGTCTTTGTCATAATCAACATACTCGACTTGTACTTCTAGCTTAAGTTTAAGACGTTC
AGTCGGGGTGAAAGATCTTTTCTTTCCGAGGTATTTGCTTTCTATTCGACTTAGCGATGA
AGTGAAGTCTCGTCCTTCGCCACCGACTTTTCTGATATTGATATCCATTACACGATCATC
CGGGCGAATCAGGTTGATAAGTATAGGAGAGATCATCCGAGT
   
Sequence Name 36-K035351-022-011-G10C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>36-K035351-022-011-G10C-3144
TTACCCTTTTTTCTGTGACTGATTGTACCATGCCTCGTTTGGCTCCTCTCTCTCTT

Last Updated on 06.12.2018 16:11