Sequences from confirmation amplicon


Confirmation sequences for line 011G10 and hit F2P9 are shown.
Line ID 011G10
Locus F2P9 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27859526
Primers used for PCR 01xh - 3144, show primer details
   
Sequence Name 18-K119080-0022-011-G10C-01xh
GenBank Accession LN494534 [GenBank]
Sequencing primer gene specific primer, 01xh: 5'-ACATCTAGGGATGTAAAAATGGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K119080-0022-011-G10C-01xh
TAATGAGTTATGGGTTGATCCAACTCATTTAATTTATTTGATGGGTTGAGTTGTTAAATG
GGTTAACCCATTTTAAATATTATTATAAACAATAATAAATAATTATCATTAGTAATTCAT
TATCATCAAATTAAGGTTATTTACGGATTCCACTTTTTACGGGTTTACGTTTTTGGCGGG
AAAATCACGGATTTACGTTTTTGGCGGGAAAATTATAGGTTTACGTTTTTGGCGGGAAAA
TTACGGGTTGATGTTTTTGGCGGGAAAATTACGGGCTTACGTTTTTGGCGGGAAAATCTC
GGGTTTACGTTTTTGGCGGGAAAATTACGAGTTTACGTTTTTTGCGGAAAAATCTCGGGT
TTACGTTGTTGGCGGGGAAATCATGGGTTTATGATTTTGGCGGAAAAATTACGGGTTTAC
GTTTTTGGCGGGAAAATTACAGGTTTACGTTTTTGGCGGGAAAATCACGGGTTTACGTTT
TTGGCGGGAAAATCACGGGTTTACGTTTTTGGCAGAAAAATCACGGGTTTATGTTTTTGG
CGGATTAATCACGGATTTACGATTTTTGGCGGGAAAATTACGGGTTTACATTTTTGGCAC
AAAAATTATGGATTTACGTTATTGGCGGGAAAATCTCA
   
Sequence Name 04-K119079-0022-011-G10C-35St
GenBank Accession LN488131 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>04-K119079-0022-011-G10C-35St
CTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGATCAGAT
TGTCGTTTCCCGCCTTCGGTTTAAACTATCAGCTGATCCAATTGAAATAGTCAACAGTCT
CACACAAACACGTGCCATCGCCATAGGCCCATAGACTACCATTAATTCTACTCAATGACT
CGTTATCACACCCACATCAATATGCAACATGTAATTTTCTTAATTCCCAAAATAAATAAG
ATGTATGTAGGGGTGTAAAAATTGGTCAAAATTCATGCGTCAACTCAACTCAACTCAATT
CATGAACTCTAATGAGTTGAAATTTTTGACTCAAATGAGTTGATGGATCAAATGAGTTGA
TGAGTCATTTGGTTTGATGAGTAAAATAAGTTGGGTTGTAATGGTTAATGATTTCAATGG
GTTACCCAATTAACCCATCAAGTTTTGTAAAACTAAATTAAACCAACTAAAATCTCTAAA
CCAATGCCAATTTAAGTTTAACCAACACATCTAAACCAATTTAATAAAATTAATATTTTT
CCAAATATCTTAAATATACAAGCGATGAAATTGAGAAAAAGTAAACTCGTAATTTTTCCA
CAAAAAAACATAAAACCGTGATTTTTCCGCCAAAAACGTAAACCCGTGATTTTTCCGCCA
AAAACGTAAACCCGTGATTTTCCCGCCAAAAACGTAAACCCGAGATTTTCCCGCCGATAA
CGTAAATCCATAAATTTTTTGCCAAAAACCTAAACCCGTAATTT
Primers used for PCR 32xl - 8474, show primer details
   
Sequence Name 49-K119268-0022-011-G10C-32xl
GenBank Accession LN507007 [GenBank]
Sequencing primer gene specific primer, 32xl: 5'-AATTGATTCATCAACCATGTACGA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K119268-0022-011-G10C-32xl
AAGATTCGACTACACATACAAATTGATCTGCCAGTTACACGTTAGGTCCATCATTTATAC
AAAACAATCTGTATCATATGGGCTCCCTAATTAATAAACATGTGTGCATAACTCTCTGAA
AGAATGAAACATCGACATCATTGTATGGGCATAGTAGTTGAAAATCCATAAAGAGATCTC
TCTACTTCACCAAATCTAATACATTTAAGTGAACAATGTTGTTTGTAATCAACTGCGACA
AAAGATACAAGAAGAAATAGAACCACTTTTGTCGAGGAAGGTGCTGAGTAGCATAGGACA
TGAGATGTGATTTCACTTTTTAAAAACCAAATCACGTTTTGTTGTTTATTAATGTTTACA
CTTATAGTATTCCATTATGATTGATTATGGAGGGAGAAATTAAGAATCAAAGATATCTTG
TGGGAATAAGTATAACTCTATTTGTTAATTGTACCAAAAAAAACCAGCTTTTCAAATAAT
CCCGTAAAATGACAAGTGGAAGGAGCCAAAGTTGAAAGTTAGTAAATAATTATGCAAATG
TTGTTGAGTCCATTATGCAAAATGTTAACAGTCTCACACAAACACGTGCCATCATATTCA
ATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAA
TATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
CA
   
Sequence Name 25-K119267-0022-011-G10C-8409
GenBank Accession LN497686 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K119267-0022-011-G10C-8409
CATTTACAATTGAATATGATGGCACGTGTTTGTGTGAGACTGTTAACATTTTGCATAATG
GACTCAACAACATTTGCATAATTATTTACTAACTTTCAACTTTGGCTCCTTCCACTTGTC
ATTTTACGGGATTATTTGAAAAGCTGGTTTTTTTTGGTACAATTAACAAATAGAGTTATA
CTTATTCCCACAAGATATCTTTGATTCTTAATTTCTCCCTCCATAATCAATCATAATGGA
ATACTATAAGTGTAAACATTAATAAACAACAAAACGTGATTTGGTTTTTAAAAAGTGAAA
TCACATCTCATGTCCTATGCTACTCAGCACCTTCCTCGACAAAAGTGGTTCTATTTCTTC
TTGTATCTTTTGTCGCAGTTGATTACAAACAACATTGTTCACTTAAATGTATTAGATTTG
GTGAAGTAGAGAGATCTCTTTATGGATTTTCAACTACTATGCCCATACAATGATGTCGAT
GTTTCATTCTTTCAGAGAGTTATGCACACATGTTTATTAATTAGGGAGCCCATATGATAC
AGATTGTTTTGTATAAATGATGGACCTAACGTGTAACTGGCAGATCAATTTGTATGTTGT
AGTCGAATCTTCTAATTCCCTCAACAACTCTTCTCGTAATGAACACTGTCGTACATGGTT
GAGAAATCAA

Last Updated on 06.12.2018 16:11