Sequences from confirmation amplicon


Confirmation sequences for line 011H04 and hit At3g07320 are shown.
Line ID 011H04
Locus At3g07320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:2333401
Primers used for PCR 01qi - 3144, show primer details
   
Sequence Name 32-K115550-0022-011-H04C-01qi
GenBank Accession LN500712 [GenBank]
Sequencing primer gene specific primer, 01qi: 5'-ATTAATGGCGGCTTACTATTTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>32-K115550-0022-011-H04C-01qi
TTTTCACAGGAGCAAGTTCTCCGGCCGGCCAGGAATCAACTATGGCCAGCTCGGAAACAA
CCTCCCGTCACCGTCCGACTCAGTCAACCTCATCAAGTCCCTAAACGCGAAACGCGTCAA
ACTCTACGACGCTAACCCAAAAATCCTCGCCGCGTTAAACGGCACCGACATTACCGTCTC
CGTCATGGTCCCAAACGAGCTCCTCGTCAACATCTCCAAATCCGCATCACTCTCCGACGA
CTGGATCCGATCCAACATCCTCCCGTTTTACCCAACCACCAAGATCCGTTACCTCCTCGT
CGGCAACGAGATCCTCTCTTTACCAGACTCTGAGCTCAAATCTTCACTCGTTCCCGCAAT
GCGCAAGATTCAATATTTCCTTAAAGTCTCTCGGCGTTAAACACTGATAGTTTAAACCGA
AGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTC
CTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAG
TGG
   
Sequence Name 05-K115549-0022-011-H04C-3144
GenBank Accession LN488482 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K115549-0022-011-H04C-3144
TCCTCTGTATAGGATGTTCATTTCATTTGGAGAGGACGGGGTACCCGGGGATCAGATTGT
CGTTTCCCGCCATCGGTTTAAACTATCAGTGTTTAACGCCGAGAGACTTTAAGGAAATAT
TGAATCTCGCGCATTGCGGGAACGAGTGAAGATTTGAGCTCAGAGTCTGGTAAAGAGAGG
ATCTCGTTGCCGACGAGGAGGTAACGGATCTTGGTGGTTGGGTAAAACGGGAGGATGTTG
GATCGGATCCAGTCGTCGGAGAGTGATGCGGATTTGGAGATGTTGACGAGGAGCTCGTTT
GGGACCATGACGGAGACGGTAATGTCGGTGCCGTTTAACGCGGCGAGGATTTTTGGGTTA
GCGTCGTAGAGTTTGACGCGTTTCGCGTTTAGGGACTTGATGAGGTTGACTGAGTCTGAC
GGTGACGGGAGGTTGTTTCCCAGCTGGCCATAGTTTATTCCTGGCCGGCCGGAGAACTTT
GCTCCTGTGGAAAAAAAAACCCCCCGGGGGAT

Last Updated on 06.12.2018 16:11