Sequences from confirmation amplicon


Confirmation sequences for line 011H05 and hit At3g23410 are shown.
Line ID 011H05
Locus At3g23410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8384098
Primers used for PCR PD21 - 8474, show primer details
   
Sequence Name 27-K036416-022-011-H05C-PD21
GenBank Accession LN498292 [GenBank]
Sequencing primer gene specific primer, PD21: 5'-CCTTTTTCAAGAACAACCACTTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K036416-022-011-H05C-PD21
GAGCTGCTACGCCTCCTCCTGAACAGACCCGACGACCACAACATCACATTTTATTCTGAT
CGCATCATGCTCCGTGTCAAGAACCGCTTCTAGGCCTTTATGAGCAAGTGATTCAAGAAG
AGTCTGCTCTGTTTCTTCCATCGTCTCCACTATCCCTTTCTCAAGTGGTCTTTCGTTATG
AGTTTCAGAAGGTTTGTTCTCATCGGGGTTAACACGATACCCTATGGCTTCCCATGCCGG
GTTTTCGCCATTTGGATTCACCTACACAAAAGAAACGACATATCCATAAATTCTTGAACA
TTTTCTATAAGTAGCTCTGTTTTTACGACAGAGTTAACATATATAATATAGGCTCTGAAT
CCGGATTTTGGTTTAGTTCACGATTCTCAAAATTTTGGATCGGTTTATTGTAACCAAATC
AGAAAATGATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGA
GTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAA
ATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTA
CGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTA
TTTCGACGTGTCTACATTCA
   
Sequence Name 19-K036416-022-011-H05C-8409
GenBank Accession LN494670 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K036416-022-011-H05C-8409
GAGCCATTTAAATTGAATCATTTTCTGATTTGGTTACAATAAACCGATCCAAAATTTTGA
GAATCGTGAACTAAACCAAAATCCGGATTCAGAGCCTATATTATATATGTTAACTCTGTC
GTAAAAACAGAGCTACTTATAGAAAATGTTCAAGAATTTATGGATATGTCGTTTCTTTTG
TGTAGGTGAATCCAAATGGCGAAAACCCGGCATGGGAAGCCATAGGGTATCGTGTTAACC
CCGATGAGAACAAACCTTCTGAAACTCATAACGAAAGACCACTTGAGAAAGGGATAGTGG
AGACGATGGAAGAAACAGAGCAGACTCTTCTTGAATCACTTGCTCATAAAGGCCTAGAAG
CGGTTCTTGACACGGAGCATGATGCGATCAGAATAAAATGTGATGTTGTGGTCGTCGGGT
CTGGTTCAGGAGGAGGCGTAGCAGCTTCTGTTCTAGCGAAATCTGGTTTAAAAGTGGTTG
TTCTTGGAAAAAG

Last Updated on 06.12.2018 16:11