Sequences from confirmation amplicon


Confirmation sequences for line 011H10 and hit At2g36200 are shown.
Line ID 011H10
Locus At2g36200 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15184304
Primers used for PCR WS03 - 8474, show primer details
   
Sequence Name 49-K017403-022-011-H10C-WS03
GenBank Accession LN506669 [GenBank]
Sequencing primer gene specific primer, WS03: 5'-GGAAGCATATTGATAGGGTTTTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K017403-022-011-H10C-ws03
ACTCATACATTGGAGTATACTTAGTATCTACATGTTGAATGTTTGTGTTTATATGTTGAA
CACAGGTGTTTGGACCATCAGCACAACAGAAGGATTTGTATGATCAAGCCGTTGTTCCAA
TTGTGAATGAAGTACTTGAAGGTTTTAATTGTACTATATTTGCGTATGGTCAGACTGGTA
CTGGTAAAACATATACAATGGAAGGAGAATGTAGAAGATCAAAGGTCTGTATATTGTTGC
AGTGTGTTTGATTTTTAAGGTTTTCTAGTGATGGTTCATTATCTAAGCTATGTACCTTTT
TTTTTATTGGGACAGAGCGCACCTTGTGGAGGTCTTCCGGCAGAAGCAGGTGTTATACCT
AGAGCTGTGAAACAAATATTTGATACGCTTGAGGGTCAGCAAGCTGAGTACAGTGTTAAA
GTCACGTTTTTGGAACTCTACAATGAAGAGATTACCGATTTGCTTGCTCCTGAAGATCTA
TCCAGAGTTGCTGCTGAAGAAAAACAAAAGAAGCCGTTGCCTCTCATGGAGGATATATTC
AATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCA
ATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
GCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTA
TTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCT
   
Sequence Name 50-K017403-022-011-H10C-8409
GenBank Accession LN507018 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K017403-022-011-H10C-8409
GGACATGAAGCCATTTACAATTGAATATATCCTCCATGAGAGGCAACGGCTTCTTTTGTT
TTTCTTCAGCAGCAACTCTGGATAGATCTTCAGGAGCAAGCAAATCGGTAATCTCTTCAT
TGTAGAGTTCCAAAAACGTGACTTTAACACTGTACTCAGCTTGCTGACCCTCAAGCGTAT
CAAATATTTGTTTCACAGCTCTAGGTATAACACCTGCTTCTGCCGGAAGACCTCCACAAG
GTGCGCTCTGTCCCAATAAAAAAAAAGGTACATAGCTTAGATAATGAACCATCACTAGAA
AACCTTAAAAATCAAACACACTGCAACAATATACAGACCTTTGATCTTCTACATTCTCCT
TCCATTGTATATGTTTTACCAGTACCAGTCTGACCATACGCAAATATAGTACAATTAAAA
CCTTCAAGTACTTCATTCACAATTGGAACAACGGCTTGATCATACAAATCCTTCTGTTGT
GCTGATGGTCCAAACACCTGTGTTCAACATATAAACACAAACATTCAACATGTAGATACT
AAGTATACTCCAATTTTTGAGTAGACGCAACTAATTATACCTTATCAAAAGTGAAAACCC
TAT

Last Updated on 06.12.2018 16:11