Sequences from confirmation amplicon


Confirmation sequences for line 012E06 and hit At1g78390 are shown.
Line ID 012E06
Locus At1g78390 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:29492682
Primers used for PCR 68gp - 3144, show primer details
   
Sequence Name 25-K111953-0022-012-E06C-68gp
GenBank Accession LN497509 [GenBank]
Sequencing primer gene specific primer, 68gp: 5'-GAGACTGGTTTGTGAAGTGGATTT-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K111953-0022-012-E06C-68gp
TCTGGTCTGTTCCAGTCACCGGAAGGTTATGCACGACAGGTTTCTCCGGTACCGGGAAGA
AGTTCCCGGCTATTTGGACGGCAGGATCCGCCGTTTTAGGATGGGGATGACGGCGGCGCT
CGTGTGAGATCATGGCGTTTTCCACCGCGTCTAAAACCATAGCGGCGGTTCTCTGCACCG
GGTTTAATTTTGGATTCTGATTCGTCGTTTTGGGGATCAAGTTGGTGACATCGTTACATA
GTTTCTTCCAAGACGGTGGTTTGAATGTCGGAGATTGGAGAGAAGACGAGACGGAAGATT
TTTTGTTGACGGAGGAAGAAACATAGCAGGGGACTACTAGTTTCTTGTTTCTAAGTGTTG
AGGAAAAAGATAGAGATTGAAGTTTAGGTCTCGAAGAGGAAGATGAAGTTGAGAAAGTTC
GGTCGAGGAATTGAGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCA
   
Sequence Name 09-K111952-0022-012-E06C-3144
GenBank Accession LN490243 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K111952-0022-012-E06C-3144
TTCGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTCTCAATTCCTCGAC
CGAACTTTCTCAACTTCATCTTCCTCTTCGAGACCTAAACTTCAATCTCTATCTTTTTCC
TCAACACTTAGAAACAAGAAACTAGTAGTCCCCTGCTATGTTTCTTCCTCCGTCAACAAA
AAATCTTCCGTCTCGTCTTCTCTCCAATCTCCGACATTCAAACCACCGTCTTGGAAGAAA
CTATGTAACGATGTCACCAACTTGATCCCCAAAACGACGAATCAGAATCCAAAATTAAAC
CCGGTGCAGAGAACCGCCGCTATGGTTTTAGACGCGGTGGAAAACGCCATGATCTCACAC
GAGCGCCGCCGTCATCCCCATCCTAAAACGGCGGATCCTGCCGTCCAAATAGCCGGGAAC
TTCTTCCCGGTACCGGAGAAACCTGTCGTGCATAACCTTCCGGTGACTGGAACAGTACCA
GAATGCATTCAAGGAGTTTACGTCAGAAACGGAGCAAATCCACATCAC

Last Updated on 06.12.2018 16:11