Sequences from confirmation amplicon


Confirmation sequences for line 012F12 and hit At5g57035 are shown.
Line ID 012F12
Locus At5g57035 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:23082925
Primers used for PCR CM58 - 8474, show primer details
   
Sequence Name 11-K104111-0022-012-F12C-CM58
GenBank Accession LN491087 [GenBank]
Sequencing primer gene specific primer, CM58: 5'-TGCTCTCAAACAGTCCATAGAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104111-0022-012-F12C-CM58
ACACTCTTCTTGAAGAGGGCGATACAGAAAGTACACAAAAGAAGAAATAGCTGCAGCTAC
AGATAATTTCTCTTCCCGTAAAATAATCGGCGAAGGAGGATATGGAAAAGTATACAAATG
CAGTCTTGATCATACCCCAGTAGCTCTTAAAGTTCTCAAACCTGATTCTGTTGAAAAGAA
AGAGGAATTCTTGAAAGAGGTAAATTACTAGTAGTCTCATGGCCTAATAACAACAGCAGC
CTTTAACACTCTGAGATCTGAAATCAACTATTTTACTTTGCAGATCTCAGTCTTAAGCCA
GCTTCGTCATCCCCATGTGGTTCTTCTCCTTGGTGCTTGTCCTGAAAATGGATGCCTTGT
CTATGAGTACATGGAAAATGGTAGCTTAGATTGTCACATCTCTCCCAAAAAAGGGAAACC
ATCTCTCTCATGGTTCAATTGGTAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCA
ATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTC
AAAAATGTAGATGTCCG
   
Sequence Name 27-K104112-0022-012-F12C-8409
GenBank Accession LN498359 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K104112-0022-012-F12C-8409
GCCTTTACAATTGAACCATGAGAGAGATGGTTTCCCTTTTTTGGGAGAGATGTGACAATC
TAAGCTACCATTTTCCATGTACTCATAGACAAGGCATCCATTTTCAGGACAAGCACCAAG
GAGAAGAACCACATGGGGATGACGAAGCTGGCTTAAGACTGAGATCTGCAAAGTAAAATA
GTTGATTTCAGATCTCAGAGTGTTAAAGGCTGCTGTTGTTATTAGGCCATGAGACTACTA
GTAATTTACCTCTTTCAAGAATTCCTCTTTCTTTTCAACAGAATCAGGTTTGAGAACTTT
AAGAGCTACTGGGGTATGATCAAGACTGCATTTGTATACTTTTCCATATCCTCCTTCGCC
GATNATTTTACGGGAAGAGAAATTATCTGTAGCTGCAGCTATTTCTTCTTTTGTGTACTT
TCTGTATCGCCCGTCTCTCAAGAAGAGTTGCTCAATGACTTTCTGTTTCTCTATGGACTG
TTTG

Last Updated on 06.12.2018 16:11