Sequences from confirmation amplicon


Confirmation sequences for line 012H10 and hit At1g12160 are shown.
Line ID 012H10
Locus At1g12160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:4126682
Primers used for PCR MS03 - 3144, show primer details
   
Sequence Name 18-K016431-022-012-H10C-3144
GenBank Accession LN494094 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K016431-022-012-H10C-3144
GGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATT
TCATTTGGAGAGGACAGGGTACCCGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAA
CTATCAGTGTTTAAACCGAAGATACTTAAGAGACTAATCAACCATTAATTAATCACACTT
TTAAGTAACCAAAATATTACTTACCAGGAATATGAGCAAGACGAGGCTCAGTGAAGTGAC
CATTGCAAACCACGACCGCGTCGAAGATTTCTTCGCCGGAGACACCATTTGAGCTCTTAA
ACTGAACTCTCCATTTACGGTTATTCTCCGGCGACGGCTCCACACACAAAACCTCTGTCT
CAAATCGGATCATATCTTCGATCTTGAACTCTTTGGCGAAATCTTGAAGGTACATCAAGA
CTTCCCTATGATCGGGGTACCGTCTCGGGTCGCCGTCACTGGATCGGGTAACGAATGGAA
AGTCACTGTAACCCATACACTCTCGTGGTAGATTGGTGCGGAGGGACTGGTAGATGCTCG
AGTGTACGATGGTTCGATCCGGGTCAACGCTAACCGAGTCTGAATCGACTCGATCAGTGC
ACACCCAG
   
Sequence Name 22-K016431-022-012-H10C-MS03
GenBank Accession LN495894 [GenBank]
Sequencing primer gene specific primer, MS03: 5'-GACTTGACACTATGACCAGCGTAA-3'
Sequence (A. th genome BLAST matches underlined) 
>22-K016431-022-012-H10C-ms03
CCTCACGTGCTCGTCACGTGGCCGTGATCGGACTAGGAGCCGCCGGCCTCGTGGCGGTTC
GAGAGCTTCGTCGTGAAGGTCACACCGTCATCGGTTTCGAGCGTGAGAAACATGTTGGTG
GACTCTGGGTGTACACTGATCGAGTCGATTCAGACTCGGTTAGCGTTGACCCGGATCGAA
CCATCGTACACTCGAGCATCTACCAGTCCCTCCGCACCAATCTACCACGAGAGTGTATGG
GTTACAGTGACTTTCCATTCGTTACCCGATCCAGTGACGGCGACCCGAGACGGTACCCCG
ATCATAGGGAAGTCTTGATGTACCTTCAAGATTTCGCCAAAGAGTTCAAGATCGAAGATA
TGATCCGATTTGAGACAGAGGTTTTGTGTGTGGAGCCGTCGCCGGAGAATAACCGTAAAT
GGAGAGTTCAGTTTAAGAGCTCAAATGGTGTCTCCGGCGAAGAAATCTTCGACGCGGTCG
TGGTTTGCAATGGTCACTTCACTGAGCCTCGTCTTGCTCATATTCCTGGTAAGTAATATT
TTGGTTACTTAAAAGTGTGATTAATTAATGGTTGATTAGTCTCTTAAGTATCTTCGGTTT
AAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTC
CTCTCCAAATGAAATGAACTTCCTTATA

Last Updated on 06.12.2018 16:11