Sequences from confirmation amplicon


Confirmation sequences for line 013B09 and hit At2g36160 are shown.
Line ID 013B09
Locus At2g36160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:15170564
Primers used for PCR 20mk - 8474, show primer details
   
Sequence Name 87-K114351-0022-013-B09C-20mk
GenBank Accession LN514790 [GenBank]
Sequencing primer gene specific primer, 20mk: 5'-CTTCTTCCTCTTCTACCACCCTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>87-K114351-0022-013-B09C-20mk
GGAGGGAGAACATCCTCTGCACAACAGTACGGTGTTAGAAATGATGAGGAAACAATACAA
AAGGGAAAATAGCTGAGATAAGACTATGGGTTATGAAGCTTACCAATACGGCCTATTTTC
ATGCCGGAACGAGCAAGGGCTCTAAGGGCAGACTGTGCTCCAGGACCAGGTGTCTTGGTC
TTGTTTCCACCTGTGGCACGGAGCTTCACATGCATGGCAGTGATACCAAGTTCCTGTGTT
TAGAATAGTAGTTTACATTCATTAAAAAGGATGAGGAATTTAGTTTTGTAATGAATGTCT
CAGGTCATATATGTAGTAAGTACCTTGCATCGCTGAGCAACATCCTGTGCTGCAAGCATA
GCTGCGTAAGGTGAGGACTCATCTCTATCAGCTTTCACCTTCATTCCACCTAATCAAAAT
CAAAAGAGGAAAGTTATCGAAATGTTTAGGACAACAAGAACTAATCAAGAGAATTGAGAC
ACTACATAACAATTCATCTAACAAGTCATATCCACAAAAGAGTATAACATTTGTGGTCTC
GTTCCACATATAGTAAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTA
TGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCANAAATG
TAGATT
   
Sequence Name 39-K114350-0022-013-B09C-8409
GenBank Accession LN503437 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K114350-0022-013-B09C-8409
ACGAGACCACAAATGTTATACTCTTTTGTGGATATGACTTGTTAGATGAATTGTTATGTA
GTGTCTCAATTCTCTTGATTAGTTCTTGTTGTCCTAAACATTTCGATAACTTTCCTCTTT
TGATTTTGATTAGGTGGAATGAAGGTGAAAGCTGATAGAGATGAGTCCTCACCTTACGCA
GCTATGCTTGCAGCACAGGATGTTGCTCAGCGATGCAAGGTACTTACTACATATATGACC
TGAGACATTCATTACAAAACTAAATTCCTCATCCTTTTTAATGAATGTAAACTACTATTC
TAAACACAGGAACTTGGTATCACTGCCATGCATGTGAAGCTCCGTGCCACAGGTGGAAAC
AAGACCAAGACACCTGGTCCTGGAGCACAGTCTGCCCTTAGAGCCCTTGCTCGTTCCGGC
ATGAAAATAGGCCGTATTGGTAAGCTTCATAACCCATAGTCTTATCTCAGCTATTTTCCC
TTTTGTATTGTTTCCTCATCATTTCTAACACCGTACTTGTTGTTGCAGAGGATGTTACTC
CCATCCCAACAGACAGTACCCGCAAAAAGGGTGGT

Last Updated on 06.12.2018 16:11