Sequences from confirmation amplicon


Confirmation sequences for line 013C04 and hit At5g45410 are shown.
Line ID 013C04
Locus At5g45410 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:18402086
Primers used for PCR 02fb - 3144, show primer details
   
Sequence Name 50-K111269-0022-013-C04C-02fb
GenBank Accession LN507208 [GenBank]
Sequencing primer gene specific primer, 02fb: 5'-CCTCATCATTATCATCAACAATGG-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K111269-0022-013-C04C-02fb
GTTTTACTATCTACACATTAGGATGCTTCAGTTCCATTTTTCGTGGGTCTTTTAGTGTTT
CTGTTTTCTATTTTTTTTTTTTTTGGGGTTTTGGTTTCCTTGGAAAACCCCCCTTTATAC
CAAAAGAAGGTAAATTTTCCGAATGGAAGAAAACCCAAAACCTTCCTTTTAATTTGAAAA
AAGGGTAAAAAGGGAAATTTTAACCCTTTTTTTAAACCCCTTTTGAAGGGGAAAAACCAC
CCCCTTGGTAGGGGTTACCCCATTCCCGGAAATAAAAAAACCCTCCCCCCACCCAAAAAT
TCCCAACCCTAACAAAAAATTTGGAATTTCCAAAAACAAAAAAAAACTTCCCCCCAAAAA
AAAACCCCTTCCAAAATTTAAAAACCCGGATGTTTAAACCCAAGGGGGGAAAACAAAATT
TTAACCCCGGGGACCCCGGCCCCCCCCAAAGAAAAGAAACTCCCTAAAATAAAAAAGGGG
CCTGCCAAAGAAAAGGGGAATGGGCCCCCCCCCCTACCCCCGGGGAAAATCAAAACCACC
CCAA
   
Sequence Name 34-K111268-0022-013-C04C-3144
GenBank Accession LN501461 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K111268-0022-013-C04C-3144
ATCCTTGCAGACCCTTCCTCTATTAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACC
CGGGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTTAAATTTATGA
ATGGACTCTGTTATTGAGAGAATGTTCTTATTGATTTCAGAAACTACAAATTACTATGTA
AGGATTGGATATTTGTGTGTGAGGGAAGGTTTTTGTAATTCAGTGAATTGTGTAAACCAT
AACAATGGTGTCGTTTTATCCCTTCAAAATGGGTCTAAATAAAAGGTTAATAATTCCCTC
TTTAACCTCTCTTACAATTCAAAAGAAAGTTATTGACTTTACTTCAATCAGAAAACTTAA
CCTCATCTAGTAATAAAGAGAGTTATACAAAGAAAACAAAAACACAAAAAAAAAAAAAAT
TAAAAAACAAAACCCTTAAAAACCCCCCAAAAAGGGAAATTAAACCCCCCAATTTTTGAA
AATAATAAAAACTTTAAAAGGGTAAAAACCCCCCTTAACTTTTTTAAAAAAAAAAAAAAA
G

Last Updated on 06.12.2018 16:11