Sequences from confirmation amplicon


Confirmation sequences for line 013C08 and hit At1g19310 are shown.
Line ID 013C08
Locus At1g19310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:6677066
Primers used for PCR 02iy - 8474, show primer details
   
Sequence Name 61-K113020-0022-013-C08C-02iy
GenBank Accession LN510567 [GenBank]
Sequencing primer gene specific primer, 02iy: 5'-CGTGACAAATTCAAGCAAATTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>61-K113020-0022-013-C08C-02iy
TTTGGTCATGAGAGTTGTTGATTAGTTGATTTAATCCTCTGTGTTTATTTGTGCTAATTT
ATATGGTGCTTGTGATAATCCAGTGTCGGAGATTAAAAGCAGAGGTTTTGTGGTGAATCA
TGTCTGATGTCCCTTCTTGTTCTAGCGGTAATGATACATTCAATGAGACTAATTTATCAG
GGTTATTGTCTCATGAGCGGATACATATTTGAATGTATTTAGAAAAATAAACAAATAGGG
GTTCCGCGCACATTTCCCCGAAAAGTGCCACCTGACGTCTAAGAAACCATTATTATCATG
ACATTAACCTATAAAAATAGGCGTATCAGGGTTATTGGCTTCATGTCCGGGAAATCTACA
TGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTT
TTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 29-K113019-0022-013-C08C-8409
GenBank Accession LN499368 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K113019-0022-013-C08C-8409
CATACCCTGATACGCCTATTTTTATAGGTTAATGTCATGATAATAATGGTTTCTTAGACG
TCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATA
CATTCAAATATGTATCCGCTCATGAGACAATAACCCTGATAAATTAGTCTCATTGAATGT
ATCATTACCGCTAGAACAAGAAGGGACATCAGACATGATTCACCACAAAACCTCTGCTTT
TAATCTCCGACACTGGATTATCACAAGCACCATATAAATTAGCACAAATAAACACAGAGG
ATTAAATCAACTAATCAACAACTCTCATTGACCAAAATGAAATTAGTGATTCCAAATTTT
CTAATTTGCTTGATTTTTGTCACG

Last Updated on 06.12.2018 16:11