Sequences from confirmation amplicon


Confirmation sequences for line 013C10 and hit At3g25780 are shown.
Line ID 013C10
Locus At3g25780 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9409257
Primers used for PCR 06kb - 8474, show primer details
   
Sequence Name 58-K113379-0022-013-C10C-06kb
GenBank Accession LN509759 [GenBank]
Sequencing primer gene specific primer, 06kb: 5'-GTCATGGATCCGAATAGAATTGTT-3'
Sequence (A. th genome BLAST matches underlined) 
>58-K113379-0022-013-C10C-06kb
AAGGATGCATGACCATCTCGTGATTGTAGTGCTATTATGTGTGTACACGGCAAATTGACA
CAACCGAACCGACCAAGTTATATATATGTCACTAAAACAGTCTGACCAACAAGAGTTTGA
AGACCTGCAGGCTACGTTTATATCTTTTGACTATTATAGTATGTTCCCATTACTTTGAGA
TCTCGTAGTACTCAATAAATATTACACGGTTTTAAGTCGATAAATAATAAATACATAAAA
TTCTCTAGCTAACGCTTTTAGACAATTCGAAACCGAGTGGAAACTACACAAAGCATCTTT
TGACATATACGCACGTGTTACTTCATCATCGTCCGGGAAAATATATTCAATTGTAAATCA
CCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCACGNTTATTA
   
Sequence Name 20-K113377-0022-013-C10C-8409
GenBank Accession LN495289 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K113377-0022-013-C10C-8409
ATTTCATTGAATATATTTTCCCGGACGATGATGAAGTAACACGTGCGTATATGTCAAAAG
ATGCTTTGTGTAGTTTCCACTCGGTTTCGAATTGTCTAAAAGCGTTAGCTAGAGAATTTT
ATGTATTTATTATTTATCGACTTAAAACCGTGTAATATTTATTGAGTACTACGAGATCTC
AAAGTAATGGGAACATACTATAATAGTCAAAAGATATAAACGTAGCCTGCAGGTCTTCAA
ACTCTTGTTGGTCAGACTGTTTTAGTGACATATATATAACTTGGTCGGTTCGGTTGTGTC
AATTTGCCGTGTACACACATAATAGCACTACAATCACGAGATGGTTCATGCATCCTTTTT
CTCAAGGAAGGACTGGTCCCACTAGTATCAACAATTCTATTCGANTCCATGA

Last Updated on 06.12.2018 16:11