Sequences from confirmation amplicon


Confirmation sequences for line 013C10 and hit At3g25790 are shown.
Line ID 013C10
Locus At3g25790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9414075
Primers used for PCR 05lh - 3144, show primer details
   
Sequence Name 34-K113916-0022-013-C10C-05lh
GenBank Accession LN501492 [GenBank]
Sequencing primer gene specific primer, 05lh: 5'-TTCATCATTTGTTAACCCATCAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K113916-0022-013-C10C-05lh
AATTTAGTGGAACAAAAAGGGCATTACGAATTAATTAAACTTTCATGAGCCCTAACAAAA
AGATATGACTACTGATATATATGTTTTAACTTAAAACCAAGAAACGCACCATGAGGGCCA
CCAAGTTGTTTAAGAGCGTTCAAGAAGCGTCTGTGTAACTCTTGCGACCAACACCGCCTA
TTCTTTCTCTGCCCTCTTCTGCCACCGCCACCGCCACTGCCACCATCGCTAGTAGTCACC
GGTGATTTTATGTTGTTGTTTTTCTTCGCTGCTTCATCGTTTATTTTTATCAACTCTACC
AGCGTCTCTGTCTTTTATTTGTAAATGGCTTCATGTCCGGGTACCCTGATAGTTTAAACT
ATCCTGAGTATTTTTAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATC
CCCGGGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGC
GAAGGATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATA
   
Sequence Name 06-K113915-0022-013-C10C-3144
GenBank Accession LN488897 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>06-K113915-0022-013-C10C-3144
CAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGGGGA
TCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTAAAAATACTCAGGATAG
TTTAAACTATCAGGGTACCCGGACATGAAGCCATTTACAAATAAAAGACAGAGACGCTGG
TAGAGTTGATAAAAATAAACGATGAAGCAGCGAAGAAAAACAACAACATAAAATCACCGG
TGACTACTAGCGATGGTGGCAGTGGCGGTGGCGGTGGCAGAAGAGGGCAGAGAAAGAATA
GGCGGTGTTGGTCGCAAGAGTTACACAGACGCTTCTTGAACGCTCTTAAACAACTTGGTG
GCCCTCATGGTGCGTTTCTTGGTTTTAAGTTAAAACATATATATCAGTAGTCATATCTTT
TTGTTAGGGCTCATGAAGTTTAATTAATTCGTAATGACCTTTTTGTTCACTAAATTTTGA
AGTAGCTACGCCGAAACAGATAAGAGATATAATGAAGGTTGATGGGTT

Last Updated on 06.12.2018 16:11