Sequences from confirmation amplicon


Confirmation sequences for line 013E02 and hit At5g49215 are shown.
Line ID 013E02
Locus At5g49215 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19955393
Primers used for PCR 01xq - 3144, show primer details
   
Sequence Name 40-K119512-0022-013-E02C-01xq
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer gene specific primer, 01xq: 5'-AAATATTTACGGGTGTGGCGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>40-K119512-0022-013-E02C-01xq
TGTCTCTGTGTGGTTTGGTAAGCGATGGTTTCGTGACGTCTCTTTTTACGAAGCAGCTCT
TTGTTATTGGGCTGGGCTTTTTAACGAGGCCCAAATATTTAAAAAAATTAATATCTGGTT
ACTGGGCTAATACAAAATTTCTTTGGTTACACATGAATGAAGAAATGAACCTGTACAAGA
TATATTAATCTCACAAGCTGCAAATAACAGGGACAACAACACCAACAAGGAACAGGTAAT
AATTCTACACACTGTGGTTTATTTTTGGGGAAAAGAAAAGAATCAGAACCATTTGCTCTC
TCTTAAACAAATATGAATCATCATTCCTAATGAAGTTTGGTTTCATAAGGCCTCAGCTCT
ACCATAGTAACTATCAAAACTCTTCAGCTCAAGGCACGGTTCAGGGACTACGAATTCCGA
AGACCCGTCAACATATGAGCATTGCCAAGAATCAGCAGGGGAAGCCGAGTTTTTAATCGA
CAGAGAGATATTCGATAACAAAATCGAAGTGAAAGGAGACTCTTTCGTTCCAAAGAAATA
TCCAGCAGTGCTTATGTTTTCTCCAACAATGTCATGTAACCTGATGTGACTCACAAGCGG
GTAAGCATTTTCATCGAAACCAGAATCCGGATGAGTACCGAAGCTACCGTTGGCTAGAAA
TGCAGTACCAACATTAACCATATGGATGTTGGAAATATCAATCTCCTTAATATAACCACC
TCTTCCATTTGTTGTTCTAAAGGCAATCCCGGATAACGAGTTGTGTATGTGAGCATCACT
GACCTCAACATCAGATATACCACCAGACATTTCACTACCAAAGGCGGGAAACGACAATCT
GATCCCCGGGTACCCTGTCCTCTCCCAATGAAATGAACTTCCTTATATAGAGGAAGGGTC
TTGCGAA
   
Sequence Name 08-K119511-0022-013-E02C-3144
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>08-K119511-0022-013-E02C-3144
CTTCGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTTGGTAGTGAAATGTCTGGTGGTATATCTGATGTT
GAGGTCAGTGATGCTCACATACACAACTCGTTATCCGGGATTGCCTTTAGAACAACAAAT
GGAAGAGGTGGTTATATTAAGGAGATTGATATTTCCAACATCCATATGGTTAATGTTGGT
ACTGCATTTCTAGCCAACGGTAGCTTCGGTACTCATCCGGATTCTGGTTTCGATGAAAAT
GCTTACCCGCTTGTGAGTCACATCAGGTTACATGACATTGTTGGAGAAAACATAAGCACT
GCTGGATATTTCTTTGGAACGAAAGAGTCTCCTTTCACTTCGATTTTGTTATCGAATATC
TCTCTGTCGATTAAAAACTCGGCTTCCCCTGCTGATTCTTGGCAATGCTCATATGTTGAC
GGGTCTTCGGAATTCGTAGTCCCTGAACCGTGCCTTGAGCTGAAGAGTTTTGATAGTTAC
TATGGTAGAGCTGAGGCCTTATGAAACCAAACTTCATTAGGAATGATGATTCATATTTGT
TTAAGAGAGAGCAAATGGTTCTGATTCTTTTCTTTTCCCCAAAAATAAACCACAGTGTGT
AGAATTATTACCTGTTCCTTGTTGGTGTTGTTGTCCCTGTTATTTGCAGCTTGTGAGATT
AATATATCTTGTACAGGTTCATTTCTTCATTCATGTGTAACCAAAGAAATTTTGTATTAG
CCCAGTAACCAGATATTAATTTTTTTAAATATTTGGGCCTCGTTAAAAAGCCCAGCCCCA
TAACAA

Last Updated on 06.12.2018 16:11