Sequences from confirmation amplicon


Confirmation sequences for line 013E06 and hit At4g31340 are shown.
Line ID 013E06
Locus At4g31340 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:15207578
Primers used for PCR 06jd - 8474, show primer details
   
Sequence Name 53-K113845-0022-013-E06C-06jd
GenBank Accession LN508217 [GenBank]
Sequencing primer gene specific primer, 06jd: 5'-TCAGAAGACAAATGAAGAGCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>53-K113845-0022-013-E06C-06jd
CATATAGTTTTTTTTCTAAATTTTAAATTTTCAGGGAGAAACGTTGAGGACAAAGCTCGA
AGCAACCACAAAGGCGAAGGAACCGCTAGAGGTATTAGTTTGACACAGTTTTACATTTGG
CTTTTCTCTGATGCTATTATAGCATTAGATTTTCATTGTGTGGGAAATTAGCGGAGCCTG
GAATCTGGAAGTCGGCAGAAAGCTAATGTCAATTGTTTTATTTACTCAGGCTCATGGTTC
GTGGCTTCCCCCTTGGCTTGCTGTTCACTGGTTTAAATTTCAGGATATATTCAATTGTAA
ATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAG
AAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATT
   
Sequence Name 26-K113844-0022-013-E06C-8409
GenBank Accession LN498016 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K113844-0022-013-E06C-8409
TGAAATTTAAACCAGTGAACAGCAAGCCAAGGGGGAAGCCACGAACCATGAGCCTGAGTA
AATAAAACAATTGACATTAGCTTTCTGCCGACTTCCAGATTCCAGGCTCCGCTAATTTCC
CACACAATGAAAATCTAATGCTATAATAGCATCAGAGAAAGCCAAATGTAAAACTGTGTC
AAACTAATACCTCTAGCAGTTCCTTCGCCTTTGTGGTTGCTTCGAGCTTTGTCCTCAACA
TTTCTTCCTGAAATTTAAAATTTAGAAAAAAACTATATGTGAGGCAAATCTACTTTAATA
AGAGGTTACTGTACCTCAGCTATCTTAATAGCACGTTCAAGTTTTCCTATTTTGTTCTTC
TGCT

Last Updated on 06.12.2018 16:11