Sequences from confirmation amplicon


Confirmation sequences for line 013F03 and hit At3g24300 are shown.
Line ID 013F03
Locus At3g24300 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8806448
Primers used for PCR DC39 - 8474, show primer details
   
Sequence Name 34-K032603-022-013-F03C-DC39
GenBank Accession LN501308 [GenBank]
Sequencing primer gene specific primer, DC39: 5'-CTTCTCTTTGGCAAACAATCCTAC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032603-022-013-F03C-DC39
CTCATGTAGTTGGGCTGCCTCGAGTGGATCATCATATTGTACAAGCTCCGCGAGCTTGTT
GCATCCGATAAGGACGACAGAAGCCATGAAGCCGCACACAATCGCTGCCCATGGCTCTAC
GACGGAGCAACCTGCGGTTATGGCCGCAAACCCACCGAGTAACCCGTTGCAAACGTCCGT
TACGTTCCAGTGGCCTGATAGGAGACGTTTACCAAAGAGTGTGGTTAACACCATCATACT
CATTGAAGAAAGAATCATTTGAAATTGATTAAACCGAATATTCAATCAATGGCGAAACGA
CAATCTACATGGATCAATTGAAGCCATCAGGATATATTCAATTGTAAATGGCTTCATGTC
CGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAA
AGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAA
CAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACTTCA
   
Sequence Name 34-K032603-022-013-F03C-8409
GenBank Accession LN501307 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K032603-022-013-F03C-8409
TTACATTGAAATATCCTGATGGCTTCAATTGATCCATGTAGATTGTCGTTTCGCCATTGA
TTGAATATTCGGTTTAATCAATTTCAAATGATTCTTTCTTCAATGAGTATGATGGTGTTA
ACCACACTCTTTGGTAAACGTCTCCTATCAGGCCACTGGAACGTAACGGACGTTTGCAAC
GGGTTACTCGGTGGGTTTGCGGCCATAACCGCAGGTTGCTCCGTCGTAGAGCCATGGGCA
GCGATTGTGTGCGGCTTCATGGCTTCTGTCGTCCTTATCGGATGCAACAAGCTCGCGGAG
CTTGTACAATATGATGATCCACTCGAGGCAGCCCAACTACATGGAGGGTGTGGCGCGTGG
GGGTTGATATTCGTAGGAT
Primers used for PCR FP45 - 3144, show primer details
   
Sequence Name 29-K032924-022-013-F03C-3144
GenBank Accession LN499182 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032924-022-013-F03C-3144
TCCACTATCCTTCGCAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGAC
AGGGTACCAGCTTTGCAATTCATACAGAAGTGAGAAAAATGGCTTCTATGATATCCTCTT
CAGCTGTGACTACAGTCAGCCGTGCTTCTACGGTGCAATCGGCCGCGGTGGCTCCATTCG
GCGGCCTCAAATCCATGACTGGATTCCCAGTTAAGAAGGTCAACACTGACATTACTTCCA
TTACAAGCAATGGTGGAAGAGTAAAGTGCATGGTGACGGTGTTCGGCATTCTGAATCTCA
CCGAGGACTCCTTCTTCGATGAGAGCCGGCGGCTAGACCCCGCCGGCGCTGTCACCGCGG
CGATCGAAATGCTGCGAGTCGGATCAGACGTCGTGGATGTCGGACCGGCCGCCAGCCATC
CGGACGCGAGGCCTGTATCGCCGGCCGATGAGATCAGACGTATTGCGCCGCTCTTAGACG
CCCTGTCCGATCAGATGCACCGTGTTTCCCGCCATTGGTAATCTACATTTTCAAATCAAT
TGATTGGTTTGAAATTGATTTTTCTTCAATTGTATTTTAGAGCTGCTGTGCATCCTGAGA
GTGTGGTGTTAACCGCTGTACGGCCGATTCCGCTCCATTGGCCGTAGTTGGAACCAGAAT
TATACGGAACGAGTATCTTAGTGAAGGAACCGGGGTTGAAACCATACCATCCAAACCATA
GGAGGAAGGTTCCTAAGACTACTAGCGAGGCAGAGTGGCCGCGCAGAGCAATAGCGCGAC
CACCTTTCTCGAACCGACCACGACGAGGACCTTCAATAAGAGCACCCCATAAACCTGCTA
TGCCACCAACCATGTGAACAACACCGGAGCCAGCAAAGTCAATGGCTCCNGTGCTAAACA
ACGATCATCCGCTGAACGAAAGGGACTGGGCCATCCATCCGGGGGACCAAACAGTGAGAG
ACAACCGGGTAACAAATCCGGTTA
   
Sequence Name 29-K032924-022-013-F03C-FP45
GenBank Accession LN499183 [GenBank]
Sequencing primer gene specific primer, FP45: 5'-GGTTCTGTTAGAGCCAAGAATACG-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K032924-022-013-F03C-FP45
CCGGAGGACTCTTCTACTATCTCTTTGGTTACGCCTTTGCCTTTGGAGGATCCTCCGAAG
GGTTCATTGGAAGACACAACTTTGCTCTTAGAGACTTTCCGACTCCCACAGCTGATTACT
CTTTCTTCCTCTACCAATGGGCGTTCGCAATCGCGGCCGCTGGAATCACAAGTGGTTCGA
TCGCAGAGAGGACTCAGTTCGTGGCTTACTTGATATACTCTTCTTTCTTAACCGGATTTG
TTTACCCGGTTGTCTCTCACTGGTTTTGGTCCCCGGATGGATGGGCCAGTCCCTTTCGTT
CAGCGGATGATCGTTTGTTTAGCACCGGAGCCATTGACTTTGCTGGCTCCGGTGTTGTTC
ACATGGTTGGTGGCATAGCAGGTTTATGGGGTGCTCTTATTGAAGGTCCTCGTCGTGGTC
GGTTCGAGAAAGGTGGTCGCGCTATTGCTCTGCGCGGCCACTCTGCCTCGCTAGTAGTCT
TAGGAACCTTCCTCCTATGGTTTGGATGGTATGGTTTCAACCCCGGTTCCTTCACTAAGA
TACTCGTTCCGTATAATTCTGGTTCCAACTACGGCCAATGGAGCGGAATCGGCCGTACAG
CGGTTAACACCACACTCTCAGGATGCACAGCAGCTCTAAAATACAATTGAAGAAAAATCA
ATTTCAAACCAATCAATTGATTTGAAAATGTAGATTACCAATGGCGGGAAACACGGTGCA
TCTGATCGGACAGGGCGTCTAAGAGCGGCGCAATACGTCTGATCTCATCGGCCGGCGATA
CAGGCCTCGCGTCCGGATGGCTGGCGGCCGGTCCGACATCCACGACGTCTGATCCGACTC
GCAGCATTTCGATCGCCGCGGTGACAGCGCCGGCGGGGTCTAGCCGCCGGCTCTCATCGA
AGAAGGAGTC

Last Updated on 06.12.2018 16:11