Sequences from confirmation amplicon


Confirmation sequences for line 014E05 and hit At3g49110 are shown.
Line ID 014E05
Locus At3g49110 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:18202251
Primers used for PCR BM66 - 8474, show primer details
   
Sequence Name 02-K104415-0022-014-E05C-BM66
GenBank Accession LN487019 [GenBank]
Sequencing primer gene specific primer, BM66: 5'-AGTAACAACTTGAGATCCCGCTTA-3'
Sequence (A. th genome BLAST matches underlined) 
>02-K104415-0022-014-E05C-BM66
ATTGAGTCAACTATTCTCACATAGAACTTACAAAGTCAACGATATCCACCACATCATGGA
GTAGAGAGTTGGAGTTCACCACTCTACAATTCAACCTGATTTGTCCTTGAGTTCCTGTAG
TTGGTGTAATATTTCCCATCCTATTCATTGCCTCCACGAATGCATTGAAGAATGTTTGTG
TGCCATCAGCATATGCTCTCACCAAGGGGATTGTGTCAGTGGCATTGGGGCTAGAGAACA
ACTCTTGGTCGCTCTGGATAAGACCTTTTTGCTCTTTAAGATTCACATAGTATTTGTTGT
CGAAAACCAAAGGCGTACGCAGATCGAAATCCACTAAGACGCTTTGATTACCATTGCGAG
GACATTGACCACGAAGAGTTTGGAGGTAAGTAGTGTTGAGTGTAGGGTCAGGTAATCCAG
TGTTGCTAAAGTTGTATAATCTGTCCATAATGAATCGACATTGATTTTTACCAAATGTGT
GAGCCCCTAACAAAAGGAAGACCATAATTAGATCAACGATTAACTTGCTAAATTACAACT
ATATCAAACAAGGTTTCATTTCTTTTTAATAATTACCGGAGAGCGCAACAAGATCAGAAG
GACGATCGAGGCCAACATTTTTGAAGTTGGCTTTAAGCTGTGGAAGTGTGAAGAATGGAG
CTGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGT
AATTACCAATTTTTTTTCAATTCAAAAATGTAGATG
   
Sequence Name 18-K104416-0022-014-E05C-8409
GenBank Accession LN494291 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K104416-0022-014-E05C-8409
CTCATTCTCACACTTCCCAGCTTAAGCCACTTCAAAAATGTTGGCCTCGATCGTCCTTCT
GATCTTGTTGCGCTCTCCGGTAATTATTAAAAAGAAATGAAACCTTGTTTGATATAGTTG
TAATTTAGCAAGTTAATCGTTGATCTAATTATGGTCTTCCTTTTGTTAGGGGCTCACACA
TTTGGTAAAAATCAATGTCGATTCATTATGGACAGATTATACAACTTTAGCAACACTGGA
TTACCTGACCCTACACTCAACACTACTTACCTCCAAACTCTTCGTGGTCAATGTCCTCGC
AATGGTAATCAAAGCGTCTTAGTGGATTTCGATCTGCGTACGCCTTTGGTTTTCGACAAC
AAATACTATGTTGAATCTTAAAGAGCANAAAAGGTCTTTATCCAGAAGCAACCAAGAAGT
TGTTCTCTAGCCCCCAATGCCACTGAACACAATCCCCTTGGTTGAGAGCATATGCCTGAG
TGGCACACAAAACATCTCTTCAATGCATTCGTGGAAGGCAATGAAATAGGAATGGAAAAT
ATTACACCAACCTACAGGAAACTCAAGGAACAAATCAGGGTTAAATTGTAAGAGTGGGTG
AACTCCCAACTCCTCTACTCCCATGAATGTGGTGGGATACTCGTTGACTTGTGTAAGTTT
CTATGTGAAGAATATGTTGAACTCAATACTCTGCGCGACCAGAACTATATGTTTAAAGAT
AAATAAGCNGGGATCTCA

Last Updated on 06.12.2018 16:11