Sequences from confirmation amplicon


Confirmation sequences for line 014G01 and hit At5g15380 are shown.
Line ID 014G01
Locus At5g15380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:4992306
Primers used for PCR MK53 - 8474, show primer details
   
Sequence Name 10-K101499-0022-014-G01C-8409
GenBank Accession LN490588 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101499-0022-014-G01C-8409
CCATTTACATTTATATACCTCTTCATCATCTGATGACAAGGAATAGAGATTATCATCATC
ATCATCATTAATGTTGATGTTCATGTCCTCTGATTCACGTAATTTGTCAACCTCCTAGAA
AAACAGTAACAACATTCTATTAAACGACATCTTATATTCTTATGCAATATGCAATTTAAT
TTTAGGGTTTACTTACCGCATAAGTTAGAAGTGCATTCGTGATCTCTCCCACATCTTCAT
CTCCTGTACATTATCAAGGAAAGATCATAAAACCTCATTAAAGTTTCCAAGTCCTAGAAA
AAAAGAACAAAGAGAATTACCGTGTTCTTGCATAGCTTTTATCACATGTTCCTCAGGAAA
CCCCATAGCAATAAAATGATTGATAACTTTCGATTTCGAAGAACTAGCTTCAGTGCTTGC
CTATACNAAGAAACAGAGAATGGAGTGTCAAGAGATTTTAACCACAAGCGAAAAAATAGA
AAACCATAAGTAACATGACAGATTGAGCAAATAGGAAACTCACCGAATAGTTAAAGAGAG
TTTCAAGAATCATCATAGGTTCCATATTTGCTCCTGCAAATTATTAACAGAATTTGATTG
ATTGACAGAATTCGGAAATGCATATCTCAAAGAACACTATCATAGATNAACTCACCAGCG
GTTTCTTCAATTGCTCTAGCANTCATTTGAGTTTGAAATCCCATCTCGACNAAGCTAGCC
ACCGTCTCATCGGAGATTCTATTTTGAATAGAGTTTGGCGA
   
Sequence Name 10-K101503-0022-014-G01C-MK53
GenBank Accession LN490589 [GenBank]
Sequencing primer gene specific primer, MK53: 5'-AATTTCCAATTTTCTCCATCTCCT-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K101503-0022-014-G01C-MK53
TCAAATAGAATCTCCGATGAGACGGTGGCTAGCTTTGTCGAGATGGGATTTTCAACTCAA
ATGATTGCTAGAGCAATTGAAGAAACCGCTGGTGAGTTTATCTATGATAGTGTTCTTTGA
GATATGCATTTCCGAATTCTGTCAATCAATCAAATTCTGTTAATAATTTGCAGGAGCAAA
TATGGAACCTATGATGATTCTTGAAACTCTCTTTAACTATTCGGTGAGTTTCCTATTTGC
TCAATCTGTCATGTTACTTATGGTTTTCTATTTTTTCGCTTGTGGTTAAAATCTCTTGAC
ACTCCATTCTCTGTTTCTTTGTATAGGCNAGCACTGAAGCTAGTTCTTCGAAATCGAAAG
TTATCAATCATTTTATTGCTATGGGGTTTCCTGAGGAACATGTGATAAAAGCTATGCAAG
AACACGGTAATTCTCTTTGTTCTTTTTTTCTAGGACTTGGAAACTTTAATGAGGTTTTAT
GATCTTTCCTTGATNATGTACAGGAGATGAAGATGTGGGAGAGATCACGAATGCACTTCT
NACTTATGCGGTAAGTAAACCCTAAAATTAAATTGCATATGGCATAAGAATATTAGATGT
CGTTTAATAGAATGTTGTTACTGTTTTTCTAGGAAGTTGACAAATTACGTGAATCAAAGG
ACATGAACATCAACATTAATGATGATGATGATGATTATCTCTATTCCTTGTCATC

Last Updated on 06.12.2018 16:11