Sequences from confirmation amplicon


Confirmation sequences for line 015D08 and hit At2g42320 are shown.
Line ID 015D08
Locus At2g42320 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17629286
Primers used for PCR 03je - 3144, show primer details
   
Sequence Name 73-K112969-0022-015-D08C-03je
GenBank Accession LN512929 [GenBank]
Sequencing primer gene specific primer, 03je: 5'-CTGTATCTGGTCTTGTTTTGGTTG-3'
Sequence (A. th genome BLAST matches underlined) 
>73-K112969-0022-015-D08C-03je
TATTCTATTTGACATGTGAGTGAATTTCAGGATCTTGATTCTTCTCAGTTGTGAGTCTAA
ACAAATGTTCTGTTGTTTTCTCTTGGTTCACATTTTTTAGGTTGACCTTCTGGTTATCAA
ACATCATAGCTTTGAGGCAGATAATTTCGCAAGCATTTGGTAGGTCTCGCATCACACAAA
TCTCTGAACCAAATGAGAGTGGTAATAGTGATTCAGGGAAGAAAACGAACCTGAGATGGA
AGAATGGTTTCCAACAGCTTTTGGAGGATTGGCAAGAAACTGAAACATTTACTACTGCAC
TTGAGAAAATCGAGTTTTGGGTATTCTCTAGAATAGTGGAGTCTGTTTGGTGGCAGGTAT
ATATATACCAAATGTCCTAACTAAAAAAATGACAATCACCTGAAGACAATAACACTATAG
AGGAAGGCGGGAAACACTGATAGTTTAAACCGAAGGCGGGAAACGACAATCTGATCCCCG
GGTACCCTGTCCTCTCCAAATGAAATGAACTTCCTTATATAGAGGAAGGGTCTTGCGAAG
GATAGTGGGATTGTGCGTCATCCCTTACGTCAGTGGAGATATCACATCAATCCA
   
Sequence Name 66-K112968-0022-015-D08C-3144
GenBank Accession LN511646 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K112968-0022-015-D08C-3144
CTTGCAGACCCTTCCTCTATATAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCGG
GGATCAGATTGTCGTTTCCCGCCTTCGGTTAAACTATCAGTGTTTCCCGCCTTCCTCTAT
AGTGTTATTGTCTTCAGGTGATTGTCATTTTTTTAGTTAGGACATTTGGTATATATATAC
CTGCCACCAAACAGACTCCACTATTCTAGAGAATACCCAAAACTCGATTTTCTCAAGTGC
AGTAGTAAATGTTTCAGTTTCTTGCCAATCCTCCAAAAGCTGTTGGAAACCATTCTTCCA
TCTCAGGTTCGTTTTCTTCCCTGAATCACTATTACCACTCTCATTTGGTTCAGAGATTTG
TGTGATGCGAGACCTACCAAATGCTTGCGAAATTATCTGCCTCAAAGCTATGATGTTTGA
TAACCAGAAGGTCAACCTAAAAAATGTGAACCAAGAGAAAACAACAGAACATTTGTTTAG
ACTCACAACTGAGAAGAATCAAGATCCTGAAATTTCACTCACATGTCAAATAGAATATGT
GTACCTCGAAACATCATTCCCACAAGATTTGGCAACCAAAACAAGCCNAGATACA

Last Updated on 06.12.2018 16:11