Sequences from confirmation amplicon


Confirmation sequences for line 015F02 and hit At5g24270 are shown.
Line ID 015F02
Locus At5g24270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:8239389
Primers used for PCR JK11 - 8474, show primer details
   
Sequence Name 19-K020608-022-015-F02C-8409
GenBank Accession LN494571 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K020608-022-015-F02C-8409
CCATGTAGATTTCCCGGACATGAATCCAGTTTGTCGTAAATCGTACAACTTGAAAGCAAC
TGCAAATTCATGAGAAAATTCTTTGTTAGTTAGCCAATTAAGAAAAGAATATCAAGTAAA
TTCACATAGAATGAATATGAAACAATTTTTGGGGCTAGAAGAATGACATTTGACTTTTTC
ATGGACCGGCGCGCTTGGATGGAAGACACCTAAGGACCGGACAAATTCACCAAACTCGAT
CACTCCATTTCGCTTCACATCAAATACATCAAATATCTGCATATTATAAAACAGAACTTT
GTTTAAATTTACATATGTTTTCAATAATGTCACTTGTTCTAGCTAGGTAGTTACTATATG
TACCCGATCAGCGAAGAGATTCCTCCGGTTCCTATTTCTGAATAAAGCCAGCTGAAATTC
TTCCTGCAACATCAAACTTTTAAACTCTAATTAAGCTATATAGATTACCTAATTGCTGTC
TACATATGGACCTTTTAGAGTGAAAGAAGAAACCTTATGAATAAGACCATCATCGATAAT
TGAGCTGCTTAGCTTCTTGAACAGTTCATACAAAGCCTCCACTTCTTCTACCGTGACTAT
TGTCCCTCCCATTACAAGTAAAATAAA
   
Sequence Name 20-K020608-022-015-F02C-JK11
GenBank Accession LN495027 [GenBank]
Sequencing primer gene specific primer, JK11: 5'-AACAGTTGAGAGAAGGGTGTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>20-K020608-022-015-F02C-JK11
ATGGGCTGCTCTGTATCGAAGAAGAAGAAGAAGAATGCAATGCGACCACCGGGATATGAG
GATCCCGAGCTTCTTGCATCCGTCACGCCATGTTAGTTTTTAGTTTAAAATCTTATTAGT
AAGACAATCTTTAAATGTTACAATAGCATGTTGTAGAGTTTGGTAAACTTTTTCAAATTT
TATTTTACTTGTAATGGGAGGGACAATAGTCACGGTAGAAGAAGTGGAGGCTTTGTATGA
ACTGTTCAAGAAGCTAAGCAGCTCAATTATCGATGATGGTCTTATTCATAAGGTTTCTTC
TTTCACTCTAAAAGGTCCATATGTAGACAGCAATTAGGTAATCTATATAGCTTAATTAGA
GTTTAAAAGTTTGATGTTGCAGGAAGAATTTCAGCTGGCTTTATTCAGAAATAGGAACCG
GAGGAATCTCTTCGCTGATCGGGTACATATAGTAACTACCTAGCTAGAACAAGTGACATT
ATTGAAAACATATGTAAATTTAAACAAAGTTCTGTTTTATAATATGCAGATATTTGATGT
ATTTGATGTGAAGCGAAATGGAGTGATCGAGTTTGGTGAATTTGTCCGGTCCTTAGGTGT
CTTCCATCCAAGCGCGCCGGTCCATGAAAAAGTCAAATGTCATTCTTCTAGCCCCAAAAA
TTGTTTCATATTCATTCTATGTGAATTTA
Primers used for PCR 53wn - 8474, show primer details
   
Sequence Name 46-K118466-0022-015-F02C-53wn
GenBank Accession LN506010 [GenBank]
Sequencing primer gene specific primer, 53wn: 5'-TATGTATGTGAGATGGAGAGTCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>46-K118466-0022-015-F02C-53wn
ATCGTTATCAGATCGGTTTATTTCCAAATCCTAGCTTACATCTCTATCTTCCTCTTTTAA
GATATTGTACAAAAATATTCGTTTTATGTATATTTATCTTTCCTTGCATGGCTTATATTA
GGAAGATACGTTTTGCAATTCCATTTCTTCCTCTTCACAAGATGAAACGAAACTTGGAAA
CGTCCTATTTATGTCCCTACATTTACCAAGAATCTTCTGATTAGTCAATTTGATTCAATG
CAACAATCAAAGCAGTTGGTTTGGATCAAAGTTGGTCACGGTGTAAATTATGAACTCACT
TTAGATATGGCAAAGTCATGTTCTTGATGAGCGATGGATTCAAGGATACAAAGTCTTTCC
ATTCATCTATATCGATTTTTCCGTCGTTTTTGCGGTCTGCTTGCACGAAAGCCTGCAAAT
ATATACCACCATTTCCTTATAAATTCTTTATCATATCATCTTCAGTATTGGAATTAATAT
CTAGTAAACAAATGTGCATCGGTGTGGGAGTGATTACCTTATCCACCATTACTTCAATCA
TATCTTCGGAAAGAACTAGTTCGGATTCGTGAAGAAGCGCTACTACCATCTCTTTCAACT
AATCAAAATGTAATTCATTATGGTATATACATGTTAGATCAACACACATAAACGTTAGTG
GAGTAAAACTGTTTAATCTGGAGGGTACCAAAACAAAGTATCCGTAAATGGACTCACTTC
TTCTCGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTNCATTCAA
AATGT
   
Sequence Name 19-K118465-0022-015-F02C-8409
GenBank Accession LN494965 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K118465-0022-015-F02C-8409
ATTTACATTGAATATATCGAGAAGAAGTGAGTCCATTTACGGATACTTTGTTTTGGTACC
CTCCAGATTAAACAGTTTTACTCCACTAACGTTTATGTGTGTTGATCTAACATGTATATA
CCATAATGAATTACATTTTGATTAGTTGAAAGAGATGGTAGTAGCGCTTCTTCACGAATC
CGAACTAGTTCTTTCCGAAGATATGATTGAAGTAATGGTGGATAAGGTAATCACTCCCAC
ACCGATGCACATTTGTTTACTAGATATTAATTCCAATACTGAAGATGATATGATAAAGAA
TTTATAAGGAAATGGTGGTATATATTTGCAGGCTTTCGTGCAAGCAGACCGCAAAAACGA
CGGAAAAATCGATATAGATGAATGGAAAGACTTTGTATCCTTGAATCCATCGCTCATCAA
GAACATGACTTTGCCATATCTAAAGTGAGTTCATAATTTACACCGTGACCAACTTTGATC
CAAACCAACTGCTTTGATTGTTGCATTGAATCAAATTGACTAATCAGAAGATTCTTGGTA
AATGTAGGGACATAAATAGGACGTTTCCAAGTTTCGTTTCATCTTGTGAAGAGGAAGAAA
TGGAATTGCAAAACGTATCTTCCTAATATAAGCCATGCAAGGAAAGATAAATATACATAA
AACGAATATTTTTGTACAATATCTTAAAAGAGGAAGATAGAGATGTAAGCTAGGATTTGG
AAATAAACCGATCTTGATAACGATATTGGACTTGCCTTGTGTACTGTATATATTTAGTTA
TTTACATAGCGACTCTC

Last Updated on 06.12.2018 16:11