Sequences from confirmation amplicon


Confirmation sequences for line 015H08 and hit At5g49220 are shown.
Line ID 015H08
Locus At5g49220 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19958319
Primers used for PCR 01vd - 3144, show primer details
   
Sequence Name 41-K118051-0022-015-H08C-01vd
GenBank Accession LN504248 [GenBank]
Sequencing primer gene specific primer, 01vd: 5'-TTGTGAATTTGTTGGTGTGTAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>41-K118051-0022-015-H08C-01vd
GCTTTCTACTTTTCACTCTCTCTCAACAGCACCTCCTCAAAGTAAGCACCCGATGACGTA
ATGGAACACAATATCCTCTTATGTCCTCGTACTTGTGATCTCTTTCTCAGTGTTGTGTAT
GTATTGGAGCAGGTGCTATGGGTTGTAGTGATTCACAACCATCCACAAAGCTACCGTTGC
CTACTTTTGGACTCGCATCTTATAAGCTGAAAGTATCTGTGTCAAACACTGATAGTTTAA
ACCGAAGGCGGGAAACGACAATCTGATCCCCGGGTACCCTGTCCTCTCCAAATGAAATGA
ACTTCCTTATATAGAGGAAGGGTCTTGCGAAGGATAGTGGGATTGTGCGTCATCCCTTAC
GTCAGTGGAGATATCACATCAATCCACA
   
Sequence Name 05-K118050-0022-015-H08C-35St
GenBank Accession LN488543 [GenBank]
Sequencing primer T-DNA primer, 35St: 5'-GTGGATTGATGTGATATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K118050-0022-015-H08C-35St
TTCGCAGACCCTTCCACTATATAAGGAAGTTCATTTCATTTGGAGAGGACAGGGTACCCG
GGGATCAGATTGTCGTTTCCCGCCTTCGGTTTAAACTATCAGTGTTTGACACAGATACTT
TCAGCTTATAAGATGCGAGTCCAAAAGTAGGCAACGGTAGCTTTGTGGATGGTTGTGAAT
CACTACAACCCATAGCACCTGCTCCAATACATACACAACACTGAGAAAGAGATCACAAGT
ACGAGGACATAAGAGGATATTGTGTTCCATTACGTCATCGGGTGCTTACTTTGAGGAGGT
GCTGTTGAGAGAGAGTGAAAAGTTAGAAAGCAGGCATCAAGATTTTGTAGTGTTGGACCA
ACCGGTATTCTGTATATTGGATACCTACACACCAACAAATTCACA

Last Updated on 06.12.2018 16:11