Sequences from confirmation amplicon


Confirmation sequences for line 016E11 and hit At5g51460 are shown.
Line ID 016E11
Locus At5g51460 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:20903224
Primers used for PCR MS24 - 8474, show primer details
   
Sequence Name 37-K016851-022-016-E11C-8409
GenBank Accession LN502429 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>37-K016851-022-016-E11C-8409
ATTCCCGGACATGAAGCCATTTACAATTGAATATATATTGTAGAAAACAAACAAAAAGCA
AGAAAATGAACCTGTTCGTAAACTGATACAGTACGGCTATGTTCATGGTTTAAAGATTCT
CCTGCGGGACTCATGATGTCCATTCCATGGCTTCCGGCGTAATAAAGTTCACTCAAATTA
ACAAACTCATACACCTAGATGAAGCAAAATGTACTATTATTACATTAGATCACACAAGAA
AATGTTCTTGATGAAGAAAATAAAACATGTAGAAAGATTACCTTATCCCGGCTTCTTCCA
CTAATGATCGCGGTAGGGAAATACTTGGCAACATTTTGCACTGCACTACGCATCTAAACA
TGTAAAAAGGCGTGAACCATATAATTCATTATGAGGATGAATCACAAATTTGATCTGTTT
TAGCAATTGAATTTAATAGAGGAAAGTTAAAAACTTACAGCACTTGACATGTAT
   
Sequence Name 38-K016851-022-016-E11C-MS24
GenBank Accession LN502808 [GenBank]
Sequencing primer gene specific primer, MS24: 5'-CTGATTGTGCATACATGTCAAGTG-3'
Sequence (A. th genome BLAST matches underlined) 
>38-K016851-022-016-E11C-ms24
CGTCCAAATGTGAATTAAATTCAATTGCTAAAACAGATCAAATTTGTGATTCATCCTCAT
AATGAATTATATGGTTCACGCCTTTTTACATGTTTAGATGCGTAGTGCAGTGCAAAATGT
TGCCAAGTATTTCCCTACCGCGATCATTAGTGGAAGAAGCCGGGATAAGGTAATCTTTCT
ACATGTTTTATTTTCTTCATCAAGAACATTTTCTTGTGTGATCTAATGTAATAATAGTAC
ATTTTGCTTCATCTAGGTGTATGAGTTTGTTAATTTGAGTGAACTTTATTACGCCGGAAG
CCATGGAATGGACATCATGAGTCCCGCAGGAGAATCTTTAAACCATGAACATAGCCGTAC
TGTATCAGTTTACGAACAGGTTCATTTTCTTGCTTTTTGTTTGTTTTCTACAATATATAT
TCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGT
CAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGT
CCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCG
TATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTG
TCTACATTCA

Last Updated on 06.12.2018 16:11