Sequences from confirmation amplicon


Confirmation sequences for line 016G11 and hit At2g25610 are shown.
Line ID 016G11
Locus At2g25610 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10901411
Primers used for PCR 04gp - 8474, show primer details
   
Sequence Name 50-K112131-0022-016-G11C-04gp
GenBank Accession LN507214 [GenBank]
Sequencing primer gene specific primer, 04gp: 5'-CTCACTGTTTATGGGGATTTGTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K112131-0022-016-G11C-04gp
AGCAGTGCGCATTGTCTGATGCTAGAACTCGACGCTCTTTGTTAAGATTCTTGTGATTGA
GATCTTTGGGAGTGCTCTCGGGTTATTTGGAGTTATCGTTGGGATTATTATGTCAGCTCA
AGCTACATGGCCTACAAAATAGAGATATATAAACTGGTAACTTATGGACTTGTACTCTAT
AGTCTATACATTCTATGATCTATTTTTCAAAGTTCCGGATAAAAATTACACTTCTTTGCT
GTTATATCTCTTGTGTAGAAGAAAAACCATGTGTGATTAAGATTCTCCTTGAGTTGAATC
CCTATTTTTTGGTAATAAGTATGATTGAACACTGCAATTGAATCACAGGATATATTCAAT
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCC
   
Sequence Name 34-K112130-0022-016-G11C-8409
GenBank Accession LN501466 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>34-K112130-0022-016-G11C-8409
CATTTCATTGAATTATCCTGTGATTCATTGCAGTGTTCAATCATACTTATTACCAAAAAA
TAGGGATTCAACTCAAGGAGAATCTTAATCACACATGGGTTTTTCTTCTACACAAGAGAT
ATAACAGCAAAGAAGTGTAATTTTTATCCGGAACTTTGAAAAATAGATCATAGAATGTAT
AGACTATAGAGTACAAGTCCATAAGTTACCAGTTTATATATCTCTATTTTGTAGGCCATG
TAGCTTGAGCTGACATAATAATCCCAACGATAACTCCAAATAACCCGAGAGCACTCCCAA
AGATCTCAATCACAAGAATCTTAACAAAGAGCGTCGAGTTCTGAGCATCAGACAATGCGC
AACTGCTTCCAATGATTCCTACACATAACCTGCAACAAACAAACAAATCCCCATA

Last Updated on 06.12.2018 16:11