Sequences from confirmation amplicon


Confirmation sequences for line 016H07 and hit At1g43620 are shown.
Line ID 016H07
Locus At1g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:16425828
Primers used for PCR 71cs - 8474, show primer details
   
Sequence Name 27-K109029-0022-016-H07C-71cs
GenBank Accession LN498424 [GenBank]
Sequencing primer gene specific primer, 71cs: 5'-AAGATGAGAGTCAAAGCAATGGAT-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K109029-0022-016-H07C-71cs
GATTCTTGAGTTGTTCCAAAGATTTGAAGAGGATTTAGGTTTTAAGATTTGAGGCTATTG
ATACCTTGTAGACAACGCTGCTACTGAGTGACAGTAAGTAGATTTGATTCAGAGTTTTGT
ACTCTTAAAGCTACTTAGAGATTAGAATGTTTCTAGCTTTGTAACAAGCTCTTGAATAAA
TATAGTTTTGAATTTTATTAATCATCCTTCAAAGTCATTCTCATTGGACCACAAACTTAT
AAAGATGAACAAACCCATAACAATAATTTTATTACATCCATTTTATGCAACTGTGCAAAA
GAAATCTAATTATATTCCTGACCATTGAATGAAATGAATTGAGTTGCAGAGACATAGATT
ATGCAACTTAAACACACGACTAAGGAACTTTGATATCACACGCCACCACATGGAAGACAA
CACTTCTTGCCAATCTGAATAAAGAACCACTGTAACAGGTCTGGTCGATCATCTTCATCT
TTTTTCTCGGACGAGGACTCGGGCAGTGGCAGCTCTGGTGGCAAATGCCTGTGGAATGCA
TCAACTGCTGCAGCTACACCATCCTCGTTCTGAAAATGGCTTCATGTCCGGGAAATCTAC
ATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTT
TTTTTTCATTCAAAAATGTAGATGTCCGCAGCG
   
Sequence Name 11-K109028-0022-016-H07C-8409
GenBank Accession LN491152 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K109028-0022-016-H07C-8409
GCCATTTTCGAACGAGGATGGTGTAGCTGCAGCAGTTGATGCATTCCACAGGCATTTGCC
ACCAGAGCTGCCACTGCCCGAGTCCTCGTCCGAGAAAAAAGATGAAGATGATCGACCAGA
CCTGTTACAGTGGTTCTTTATTCAGATTGGCAAGAAGTGTTGTCTTCCATGTGGTGGCGT
GTGATATCAAAGTTCCTTAGTCGTGTGTTTAAGTTGCATAATCTATGTCTCTGCAACTCA
ATTCATTTCATTCAATGGTCAGGAATATAATTAGATTTCTTTTGCACAGTTGCATAAAAT
GGATGTAATAAAATTATTGTTATGGGTTTGTTCATCTTTATAAGTTTGTGGTCCAATGAG
AATGACTTTGAAGGATGATTAATAAAATTCAAAACTATATTTATTCAAGAGCTTGTTACA
AAGCTAGAAACATTCTAATCTCTAAGTAGCTTTAAGAGTACAAAACTCTGAATCAAATCT
ACTTACTGTCACTCAGTAGCAGCGTTGTCTACAAGGTATCAATAGCCTCAAATCTTTAAA
ACCTAAATCCTCTTCAAATCTTTTGTGAACAACTTCAAGGAATTTCAGAACATTGGCCAA
TGATCCATTGCTTTGACTCTCATCT

Last Updated on 06.12.2018 16:11