Genotyping and primer details


Genotyping and primer details for 016H07 - At1g43620
Line ID 016H07
Locus At1g43620 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:16425828 of line 016H07 Chr1:16425828
   
Insertion allele amplicon 8474 - 71cs
T-DNA primer 8474: 5'-ATAATAACGCTGCGGACATCTACATTTT-3'
Locus specific primer 71cs: 5'-AAGATGAGAGTCAAAGCAATGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:16415889
   
wt amplicon (over InsPos) 15dw - 71cs
wt amplicon start Chr1:16415889
wt amplicon end Chr1:16426259
wt amplicon size 1048 bp
Locus specific primer 71cs: 5'-AAGATGAGAGTCAAAGCAATGGAT-3'
2nd locus specific primer 15dw: 5'-TCTTTCATACTCATCACCGAAGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. In general, we used 8474 in the PCR and 8409 for sequencing. See the confirmation strategy page for details. For genotyping in the context of DUPLO, we used 8409 as T-DNA primer in PCRs. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11