Sequences from confirmation amplicon


Confirmation sequences for line 017A06 and hit At4g00530 are shown.
Line ID 017A06
Locus At4g00530 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:233151
Primers used for PCR DC50 - 8474, show primer details
   
Sequence Name 43-K032604-022-017-A06C-8409
GenBank Accession LN504739 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K032604-022-017-A06C-8409
GATTCTGTGAGCATTCATCTTTGGACAAAGGGAGAGCCATCTGGTAAAGAACCATCTGAC
ACTGCTAAATGAACATTGTTAGCTAGAAATGTTTGTCAATCTACTTTTGTGATCAACAAC
TATGGTTTAACCCTTTACTTTGACTTTGTTTCCAGTAACAAGTCTTAATCTCTTCCCTAG
TACTTGCACAATCAAATATCATGTTGTGAATGTTTTGAATTCAAACGACACCAGGTNACA
TGC
   
Sequence Name 43-K032604-022-017-A06C-DC50
GenBank Accession LN504740 [GenBank]
Sequencing primer gene specific primer, DC50: 5'-CCCCTCATATCAGTTTGCTGTATT-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K032604-022-017-A06C-DC50
AGGTACCTGGTGTGTTGGAAGAGCAAGACTCTGAAGAGAAATGGATTCAGCTCAAGGAAA
GAATGTCGAGGAACGCTGCGAATATACCTAAAGTTGTAAAGAGAATGCGAGAATGTATAG
AGAGTATCGACAAACTCGATTCTTTGGAAGTTACCATTCACCCGGCATTCAAAAGGCAAA
GAATTAACTGACTATTTCTCCTTCATGCTTAGGCTCTCCATAGTATTTTCTTAGGAAAGA
TATGTGTGCTGATTTGTGAATATGTAATTCTAGATTGATCTGGCATGTACATTGGTGTCG
TTTGATTCAAAACATTCACAACATGATATTTGATTGTGCAAGTACTAGGGAAGAGATTAA
GACTTGTTACTGGAAACAAAGTCAAAGTAAAGGGTTAAACCATAGTTGTTGATCACAAAA
GTAGATTGACAAACATTTCTAGCTAACATGTTCATTTAGCAGTGTCAGATGGTTCTTTAC
CAGATGGCTCTCCCTTTGGTCCAAAGATGAATTGCTTCACAGAATCTTGTGCTGCTAATA
GGACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCA
ATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATT
TTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTAT
TCTAATTCGGAAATCTTATNTCGACGTGTCTAC
Primers used for PCR DC51 - 8474, show primer details
   
Sequence Name 49-K032605-022-017-A06C-8409
GenBank Accession LN506738 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K032605-022-017-A06C-8409
ATTGAATATATCCTGAATATGGAGAAAATACCATTAAGACCTAAATGGCTGAGAAAACGC
ACCCAATGTTCAATAACTAACATGAACAGATGTAACATAACCCACCAATAACTAAACCTG
AATCATAACACAAACAATCGATCGATCAAGCAATTAGTTCCACATTCGTATTACCAAAAC
TTCAACTAGAAGAAGTATCATAGAGGAAGATAAATACTAAGAAGAATCACCCTAAATCGA
TCTTCTGATTCCAAATTTTGTCAAAAACTAATCAGAAACGAGTTTCATTATCCAGATTTT
CATGGAAACTAAATAATCAAATTCGCAGAAAGGAGAAAATGGGGGAACTAACGGGATCAA
GAACGTTGGGTTCGGGACCGTAATCGCCGGTTAAGAGAAAGTAGGAA
   
Sequence Name 49-K032605-022-017-A06C-DC51
GenBank Accession LN506739 [GenBank]
Sequencing primer gene specific primer, DC51: 5'-GACTGCTGTTGCTTCCTACTTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K032605-022-017-A06C-DC51
GACCACGTTCTTGATCCCGTTAGTTCCCCCATTTTCTCCTTTCTGCGAATTTGATTATTT
AGTTTCCATGAAAATCTGGATAATGAAACTCGTTTCTGATTAGTTTTTGACAAAATTTGG
AATCAGAAGATCGATTTAGGGTGATTCTTCTTAGTATTTATCTTCCTCTATGATACTTCT
TCTAGTTGAAGTTTTGGTAATACGAATGTGGAACTAATTGCTTGATCGATCGATTGTTTG
TGTTATGATTCAGGTTTAGTTATTGGTGGGTTATGTTACATCTGTTCATGTTAGTTATTG
AACATTGGGTGCGTTTTCTCAGCCATTTAGGTCTTAATGGTATTTTCTCCATATTCAGGA
TATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATG
ATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTA
GATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATC
CGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCG
ACGTGTCTACATT

Last Updated on 06.12.2018 16:11