Sequences from confirmation amplicon


Confirmation sequences for line 017A08 and hit At3g24480 are shown.
Line ID 017A08
Locus At3g24480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:8901466
Primers used for PCR MJ45 - 8474, show primer details
   
Sequence Name 11-K029909-022-017-A08C-8409
GenBank Accession LN490984 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029909-022-017-A08C-8409
CCATTTACAATCCACCGAAGGCCGTGACAAGAAAGCTGAACATTGCCGTGAAGATCTCTG
AGCAGGTCTTCCGGGCAAACAATTTCTCCGATCATCAAACTCAGACAACCTCAAACACAC
AGGAGCTTCACCAGTAAAGAAATTATAACTATAAGTGAAATTCTCAAGCTTAGGAAGCTG
ACAAATACTCGCCGGAATCTTCCCAGATAACAAATTATGAGCAACATTCAACTGCTCAAC
CTCCACCATACCACCAACACTCTCCGGCAACGGACCAACAAGCTCATTAAAACTAACATC
AAACACCGTCACATTCTTCAACCTTCCGATATCCGCCGGTAAACAAGAATCAAAACCA
   
Sequence Name 11-K029909-022-017-A08C-MJ45
GenBank Accession LN490985 [GenBank]
Sequencing primer gene specific primer, MJ45: 5'-AATGGTTTGAATTCTTGTTTACCG-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K029909-022-017-A08C-MJ45
ATGTTAGTTTTATGAGCTTGTTGGTCCGTTGCCGGAGAGTGTTGGTGGTATGGTGGAGGT
TGAGCAGTTGAATGTTGCTCATAATTTGTTATCTGGGAAGATTCCGGCGAGTATTTGTCA
GCTTCCTAAGCTTGAGAATTTCACTTATAGTTATAATTTCTTTACTGGTGAAGCTCCTGT
GTGTTTGAGGTTGTCTGAGTTTGATGATCGGAGAAATTGTTTGCCCGGAAGACCTGCTCA
GAGATCTTCACGGCAATGTTCAGCTTTCTTGTCACGGCCTTCGGTGGATTGTAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTACATT

Last Updated on 06.12.2018 16:11