Sequences from confirmation amplicon


Confirmation sequences for line 017A09 and hit At3g25840 are shown.
Line ID 017A09
Locus At3g25840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:9456647
Primers used for PCR 69gp - 8474, show primer details
   
Sequence Name 43-K111736-0022-017-A09C-69gp
GenBank Accession LN504881 [GenBank]
Sequencing primer gene specific primer, 69gp: 5'-GGCTCTATCCCGTAAATAATCTCC-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K111736-0022-017-A09C-69gp
CTGCGCTCTCGTCCTTCTCTTTTCGTCTTTCCGGTCAGACTCTATCTCCCTTCTCTTCTC
TCGTTCTCTTTCCCTCCTCCGATCACTATCTTTATCTCTCCGCTTACTTCCTTCCGAATC
CCTATCTCTTATGCTTCCCTCCCTGTCCATATCTCTCTCCTTACTTCTATCTCTTTCCAA
GACTTCTCGACTATATTGCCTGTCATTTTCCTCATATCGCCCACGTCGATGATATCTTTC
CTCAGTCCTAACTGAGTCAACTCTGTCATGATGATAATCCCTACTAGAATCATGATGTCT
TCCTCTGTCCGAAAACTCATCCTCCGCAAACCTTGACCTAGAAGTTTCTCTCTCCCGATC
ATGTGACTTAGATCTACTCCGGGCACGGCCTTCGTTAGACTGCCTGCTGTTTGACGGAGA
AAACGAACTTCTGCCATTATCATAACTTAATTTACTAACTCTCTCTGAAGGACCTAAGAC
CTGGAAATATATTCAATACTGGAAACCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCG
   
Sequence Name 35-K111735-0022-017-A09C-8409
GenBank Accession LN501879 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>35-K111735-0022-017-A09C-8409
TTTTCCGGTCTTAGGTCCTTCAGAGAGAGTTAGTAAATTAAGTTATGATAATGGCAGAAG
TTCGTTTTCTCCGTCAAACAGCAGGCAGTCTAACGAAGGCCGTGCCCGGAGTAGATCTAA
GTCACATGATCGGGAGAGAGAAACTTCTAGGTCAAGGTTTGCGGAGGATGAGTTTTCGGA
CAGAGGAAGACATCATGATTCTAGTAGGGATTATCATCATGACAGAGTTGACTCAGTTAG
GACTGAGGAAAGATATCATCGACGTGGGCGATATGAGGAAAATGACAGGCAATATAGTCG
AGAAGTCTTGGAAAGAGATAGAAGTAAGGAGAGAGATATGGACAGGGAGGGAAGCATAAG
AGATAGGGATTCGGAAGGAAGTAAGCGGAGAGATAAAGATAGTGATCGGAGGAGGGAAAG
AGAACGAGAGAAGAGAAGGGAGATAGAGTCTGACCGTGAAAGACGAAAAGAGAAGGAACG
AGAGCGCAGCATTGATAGGGATAGGAGAAAGGAGAGGGAAGGAGATTATTTACGGGANTA
GAGC

Last Updated on 06.12.2018 16:11