Sequences from confirmation amplicon


Confirmation sequences for line 017D04 and hit At5g55730 are shown.
Line ID 017D04
Locus At5g55730 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:22559463
Primers used for PCR CM63 - 8474, show primer details
   
Sequence Name 10-K104111-0022-017-D04C-CM63
GenBank Accession LN490627 [GenBank]
Sequencing primer gene specific primer, CM63: 5'-CAGTAGAGTTTTACCGTCGGAGAC-3'
Sequence (A. th genome BLAST matches underlined) 
>10-K104111-0022-017-D04C-CM63
TCGGCGGAATGATCTTACGGAATATGTCGGCTCATGGATGCAAAGTGTTTGCTGAGACTC
TTCTCACTAACCCTGGAGCTTCAAAAACCTATCAGGTACTATACTAAACCCCTGATTAGT
AATTAATTACGATAATTAGTAATCTTCTGTTTAATTAATCAATAATTATATGCATGTGGC
TGTGTACTAATTAATAAATGTAATTGGTACACTTATCACTACTAACCCTGATTTTAGTAA
AAGTATTAGTTTTCTGTTTTCCATTTATAACATAAATAATCAATAAGTAGATGCATGTGT
CTGTATAAATTTAGAAATTATTAAAAAAAGAAATCAGATATTTACTACTTACATAATTAA
CACTGATTAGTAGCTCTATCTAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGAT
CAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTC
AATTCAAAAATGTAGATGTCCGCAG
   
Sequence Name 26-K104112-0022-017-D04C-8409
GenBank Accession LN497896 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104112-0022-017-D04C-8409
GCCATTTACATTAGATAGAGCTACTAATCAGTGTTAATTATGTAAGTAGTAAATATCTGA
TTTCTTTTTTTAATAATTTCTAAATTTATACAGACACATGCATCTACTTATTGATTATTT
ATGTTATAAATGGAAAACAGAAAACTAATACTTTTACTAAAATCAGGGTTAGTAGTGATA
AGTGTACCAATTACATTTATTAATTAGTACACAGCCACATGCATATAATTATTGATTAAT
TAAACAGAAGATTACTAATTATCGTAATTAATTACTAATCAGGGGTTTAGTATAGTACCT
GATAGGTTTTTGAAGCTCCAGGGTTAGTGAGAAGAGTCTCAGCAAACACTTTGCATCCAT
GAGCCGACATTATTCCGGTAAGATTCATCTCCGCCGGAGCAGGAGTCGGAGCCGCCGCAG
TCTCCGACGGTAAACCTCTACT

Last Updated on 06.12.2018 16:11