Sequences from confirmation amplicon


Confirmation sequences for line 017D06 and hit At5g48060 are shown.
Line ID 017D06
Locus At5g48060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:19477302
Primers used for PCR DC52 - 8474, show primer details
   
Sequence Name 60-K032734-022-017-D06C-DC52
GenBank Accession LN510171 [GenBank]
Sequencing primer gene specific primer, DC52: 5'-GGAAAGAAGATCTTGTCTTTGTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K032734-022-017-D06C-DC52
GACCGCGTTCACACGTCTAAAGATGAAGTAATTGGCAAGATTACCCTGCCGATGAACGTG
TTCGAAAAGCGTTTAGACCACCGTCCAGTCCATTCCCGTTGGTTCAATCTTGATAAGTAC
GGTACCGGAGTTCTTGAGCCAGACGCAAGAAGAAAAGAACATAAGTTCTCGAGCCGGATT
CACCTACGAATCTGTCTTGAAGGAGGCTACCATGTGATGGACGAGTCAACAATGTACATT
AGCGACACAAGACCTACAGCTAGACAGTTATGGAAGCAGCCGGTTGGAATGTTAGAGATT
GGGATTCTTGGAGCAAATGGGTTGGTGCCAATGAAGTTAAAAGACGGTAGAGGAAGCACT
AACGCTTATTGTGTTGCTAAGTATGGTCAAAAATGGGTGAGAACCAGAACCATTCTTGAC
ACATTAAATGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCG
TCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGAC
GTGTCTACTT
   
Sequence Name 28-K032734-022-017-D06C-8409
GenBank Accession LN498735 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>28-K032734-022-017-D06C-8409
TGTGTCAAGAATGGTTCTGGTTCTCACCCATTTTTGACCATACTTAGCAACACAATAAGC
GTTAGTGCTTCCTCTACCGTCTTTTAACTTCATTGGCACCAACCCATTTGCTCCAAGAAT
CCCAATCTCTAACATTCCAACCGGCTGCTTCCATAACTGTCTAGCTGTAGGTCTTGTGTC
GCTAATGTACATTGTTGACTCGTCCATCACATGGTAGCCTCCTTCAAGACAGATTCGTAG
GTGAATCCGGCTCGAGAACTTATGTTCTTTTCTTCTTGCGTCTGGCTCAAGAACTCCGGT
ACCGTACTTATCAAGATTGAACCAACGGGAATGGACTGGACGGTGGTCTAAACGCTTTTC
GAACACGTTCATCGGCAGGGTAATCTTGCCAATTACTTCATCTTTAGACGTGTGAACGCG
GTCTTCGACAGAGATCACTAGCTGCTCTTCAAATGGCTCAGCAACCACAAAGACAA

Last Updated on 06.12.2018 16:11