Sequences from confirmation amplicon


Confirmation sequences for line 018A05 and hit At3g61760 are shown.
Line ID 018A05
Locus At3g61760 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22862801
Primers used for PCR DC56 - 8474, show primer details
   
Sequence Name 45-K032604-022-018-A05C-8409
GenBank Accession LN505428 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K032604-022-018-A05C-8409
TGAAGCCATTACATTGAAATATCCTCTAAAGGTCGTGTGTTCAAGCAAATAACAAAGATT
GATCATTCACCTTTTGACAGCCCAACTGCATCATACTTGCTATTTCCCCTGCAAATCAGG
ATCTTGCAACATCTGATGCCATTAAGATCTCTCGTGAAGTTGATCCCAAAGGTGCCAGAT
TCTTCCTTTTTTATATTTTGATACAACTGCTTTCTGAATTCACTTTGAGCTTCTTTTTTG
TGAGATATAAGCGACTAGGACATGGTAAATATGGTTTAAGTTCTATGGAGCATTGTATAT
ATGATTGATTAGCATCATCAATGCTTAGATGATGATAGTATTTCGAATTGATTGATTCTT
GTTGTTTAATGTTTGCTTCGCTGCACTTTATGCTATCAGGAGACAGGACTTTTGGAGTTT
TAACAAAAATTGATCTCATGGACCAAGGCACTAATGCTGTTGATGTAAGTTTACATTCAG
ATGATAGTAATACATTCTACATATCATTAGTCAAGATTATCGTCTAATCTGCAAGCAATC
TATCTCTAACTGCATTATGAAAGGACACAATGAAGATAAATATGAGAATCTCACTAAAGA
ATGGTTAGTGAATATTTAACAGTCCCTAAGTATTCTAAATGTTTGGAGATTTCAGATACT
TGAAG
   
Sequence Name 45-K032604-022-018-A05C-DC56
GenBank Accession LN505429 [GenBank]
Sequencing primer gene specific primer, DC56: 5'-AATTTGTATCCCCTTCCTTCAAGT-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K032604-022-018-A05C-DC56
TTAGGGACTGTTAATATTCACTAACCATTCTTTAGTGAGATTCTCATATTTATCTTCATT
GTGTCCTTTCATAATGCAGTTAGAGATAGATTGCTTGCAGATTAGACGATAATCTTGACT
AATGATATGTAGAATGTATTACTATCATCTGAATGTAAACTTACATCAACAGCATTAGTG
CCTTGGTCCATGAGATCAATTTTTGTTAAAACTCCAAAAGTCCTGTCTCCTGATAGCATA
AAGTGCAGCGAAGCAAACATTAAACAACAAGAATCAATCAATTCGAAATACTATCATCAT
CTAAGCATTGATGATGCTAATCAATCATATATACAATGCTCCATAGAACTTAAACCATAT
TTACCATGTCCTAGTCGCTTATATCTCACAAAAAAGAAGCTCAAAGTGAATTCAGAAAGC
AGTTGTATCAAAATATAAAAAAGGAAGAATCTGGCACCTTTGGGATCAACTTCACGAGAG
ATCTTAATGGCATCAGATGTTGCAAGATCCTGATTTGCAGGGGAAATAGCAAGTATGATG
CAGTTGGGCTGTCAAAAGGTGAATGATCAATCTTTGTTATTTGCTTGAACACACGACCTT
TAGAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAAT
GAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAA
AAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAAT
TACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTC

Last Updated on 06.12.2018 16:11