Sequences from confirmation amplicon


Confirmation sequences for line 018F08 and hit At1g28570 are shown.
Line ID 018F08
Locus At1g28570 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:10043508
Primers used for PCR 01gt - 8474, show primer details
   
Sequence Name 52-K112877-0022-018-F08C-8409
GenBank Accession LN507871 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>52-K112877-0022-018-F08C-8409
ATTTCATTGAATTATGCAGACTTGTTTTTTGCTTCATGTTGAAAATTTTGTGAGAAGAAA
CCAAAAGATCTTTGGCTCTTTCTTATGTAACTCTTCCCCTGTCTTATTGCTTGTGTGTAA
CTCTTCCTTTAACTTCTTCTTCTTCTTGATGCTTTTGCTTGAGTAACAAGTTTGATTGTT
GAAAACCTAGGATCATCTAAATAAATACAGAGAAGCCTTATTGATGATTTCTCAACTTAT
AGTTCTTTTCTTTGGTTCTGTTCACAGCTGAATTTTTGGGTTTTCCGCTTGTGCCTCCTT
TTTACGGATCTCAAAATGCAAATTTTGAGAAAGGAGTTAATTTTGCGGTTGGAGGAGCAA
CGGCACTGGAACGTTCCTTTCTTGAAGAGAGAGGCATTCATTNTCCTTAC
   
Sequence Name 89-K112878-0022-018-F08C-01gt
GenBank Accession LN514967 [GenBank]
Sequencing primer gene specific primer, 01gt: 5'-TGTAAGGAAAATGAATGCCTCTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>89-K112878-0022-018-F08C-01gt
CCGCAAATTACTCCTTTCTCAAATTTGCATTTTGAGATCCGTAAAAAGGAGGCACAAGCG
GAAAACCCAAAAATTCAGCTGTGAACAGAACCAAAGAAAAGAACTATAAGTTGAGAAATC
ATCAATAAGGCTTCTCTGTATTTATTTAGATGATCCTAGGTTTTCAACAATCAAACTTGT
TACTCAAGCAAAAGCATCAAGAAGAAGAAGAAGTTAAAGGAAGAGTTACACACAAGCAAT
AAGACAGGGGAAGAGTTACATAAGAAAGAGCCAAAGATCTTTTGGTTTCTTCTCACAAAA
TTTTCAACATGAAGCAAAAAACAAGTCTGCATATATTCAATTGTAAATGGCTTCATGTCC
GGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTA
ATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATA

Last Updated on 06.12.2018 16:11