Sequences from confirmation amplicon


Confirmation sequences for line 019B03 and hit At1g61480 are shown.
Line ID 019B03
Locus At1g61480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:22682530
Primers used for PCR 01hk - 8474, show primer details
   
Sequence Name 64-K113193-0022-019-B03C-01hk
GenBank Accession LN511240 [GenBank]
Sequencing primer gene specific primer, 01hk: 5'-GCCTTTCTCTTTTTGTGATACTGG-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K113193-0022-019-B03C-01hk
AGTGAACATACCGGTACACACCGATGATCTTACTATCTTTCTATTGATTTCGAGTTTTTT
GTTTATTCATTTGCTAGCTAATGCATGGTAACACACTGACTATTATTGAAAGTCTAGTTT
CAGCGAAGATATCAAAGATTGCTTCAAAAGAGGCATGGAATAATGATCTAGAGCCACAAG
ATGTCTCAGGTTTAAAGTTCTTTGAGATGAATACCATTCAAACTGCCACTGATAATTTCA
GTCTCTCAAATAAACTAGGACAAGGTGGATTTGGCTCAGTTTACAAGGTAGGAGATTTTT
TTTCTTTTCTTTGCTTCTATGTATTCGCCTTTATGTTCACGAGATGACTTTCTGCAATCT
GTTTTAGGGAAAGCTGCAAGATGGGAAAGAAATTGCTGTAAAACGGCTTTCTAGCAGCAA
AACTACCAATTATTGAAGAAAATATCCGGGAAATCTACATGGATCAGCAATGAGTATGAT
GGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGA
TGTCCG
   
Sequence Name 27-K113192-0022-019-B03C-8409
GenBank Accession LN498469 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K113192-0022-019-B03C-8409
TCGGATTTTCTTCATATTGGTAGTTTTGCTGCTAGAAAGCCGTTTTACAGCAATTTCTTT
CCCATCTTGCAGCTTTCCCTAAAACAGATTGCAGAAAGTCATCTCGTGAACATAAAGGCG
AATACATAGAAGCAAAGAAAAGAAAAAAAATCTCCTACCTTGTAAACTGAGCCAAATCCA
CCTTGTCCTAGTTTATTTGAGAGACTGAAATTATCAGTGGCAGTTTGAATGGTATTCATC
TCAAAGAACTTTAAACCTGAGACATCTTGTGGCTCTAGATCATTATTCCATGCCTCTTTT
GAAGCAATCTTTGATATCTTCGCTGAAACTAGACTTTCAATAATAGTCAGTGTGTTACCA
TGCATTAGCTAGCAAATGAATAAACAAAAAACTCGAAATCAATAGAAAGATAGTAAGATC
ATCGGTGTGTACCGGTATGTTTCACTTTGTATCTCAAGAAACAAAACGCAGCANAAGCCA
GTATCACAAAAAGAGAAAGG

Last Updated on 06.12.2018 16:11