SimpleSearch - Line and FST details
Line specific information
| Line ID | 019B03 |
| Vector Used | pAC106 |
| Line Availability | available as T3 set from NASC (N401743) |
| Segregation Analysis | 50:50:40 |
| Confirmed for Hit | At1g61480 |
| Parent of DUPLO pair | none |
| Parent of pair(s) | 8824, 10551, 10552, 10558, 10560, 10574, 10577, 10587, 11400, 11408, 94808, 94809 |
| Note |
Gene hit F10B6
| Sequence (A. th genome BLAST matches underlined) | >87-K017012-022-019-B03T-8409 AAGACTCTACGGTAAACGAGAACAATTTTTTTACGATCTTGTATAAGACGTTGATTATGA CGATCATAGTGAATATGCATTTATGAGAATCTCAATGTGATATATGAAAAGAAAAGGAAA CCTATGGGATCCTCCCCTATAGTGAGATCGAGAGCGCCAAGTGGGCCATTTTTGGTAAGC ATAACTGGCGATGCCGGATCGACCCGGAACTCCGGGTTACACGTGCCCAACTGCACGCTA CCCTATGGGATCCTCCCTATATTGAGCGAGAAAGGCAAAGCCCTATGGGATCCTCCCTAT AGTGAG |
| GenBank Accession | FR800577 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:5154058 - go to primer design |
| BLAST e Value | 4e-44 |
| Hit Clone Code (BAC ID) | F10B6 |
| Insertion Classification | Genome Hit |
| Confirmation Status | unknown |
Gene hit At3g22520
| Sequence (A. th genome BLAST matches underlined) | >18-K013491-022-019-B03-8409 TTTTGATCCATGTACACTTTCCCGGACATGAAGGCCTTTACAATTGAATATATCACTGTA GATCGCGTATGAAGTAGAGATTTGGACGAAACAACATGAGGAAACCACACATCTTGGGAA AAAACAGAGTCTTTCTGATGGTGGGAAAGAAAGTTCCGGCTATATCTGACTTTTGGTTGC TCTCGTGGTTGTTCCTATATTGCCTGATGGAAGTGTTGATAAAGGATTGGGCCGATCATA TTAATTCCTAAACTGATATGGGGCCTGATGAGCTACAACCACACGTTTTGGTAGATATAA TGAAGCCAAAAGCTCGTTTGAAACGCAACCTATGGGATCCTCCTTAAGNTGAGANGGATA TCCTGTGTCTGAATCTTTGCTTTGATTTCTTGAACAACTTAGACCTTTTGATGGGTTTAT GTCCGGAAATGAGACCAAATTAGTGGTTAGCTGATATCTGTTCAAAATTCTGACCTAAGC ACAAAAAGTTCTTGAGTTTAGATGAAAGAAGATTAAATCGCTCAGAGGAACATTTTTGCC TTTGGGAATGGGGTGAAACTTACGGTGAGCAAAAGTTAGGGTTCTTATTCTAATTGCTGC TACCCTAAAAGGATGTGTCTTTAGGGTTTGAAGGCTCCCTATTTGGCTATTCACTATGCC |
| GenBank Accession | AL753037 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr3:7974815 - go to primer design |
| BLAST e Value | 2e-129 |
| Hit Clone Code (BAC ID) | F16J14 |
| Hit Gene Code | At3g22520 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | spindle assembly abnormal protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | failed, line belongs to a contamination group, please check line 017E03 |
Gene hit At2g23780
| Sequence (A. th genome BLAST matches underlined) | >18-K013160-022-019-B03-8409 TTGATCCATTGTAGATTTCCCGGACATGAAGCACTTTACAATTGAATATATCCTGTAGAT CCCTAAAAAGTAGAGATTTGGACGAAACAAAAGAGGAAACCAGACATCTTGGGAAAAAAC AGAGTCTTTCTGTTGTTGGGAAAGAAAGTTCCAGGCTATATCTGACTTTTGGTTTTTCTC GTTTTTTTTCCATATATTTTCTTTGGAAGTTTTGATTAATTATTGGGCCGATTATATTAA TTTCTAAACTGATATTGGGCCTGATTAGCTACAACAACACATTTTGGTAGATAAAATGAT GCCAAAAGCTCGTTTTAAACGTAAACTATGGGATCCTCCCTATAGTGAGTC |
| GenBank Accession | AL753035 [GenBank] |
| Graphic View |
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| Predicted Position of Insertion | Chr2:10124534 - go to primer design |
| BLAST e Value | 2e-135 |
| Hit Clone Code (BAC ID) | F27L4 |
| Hit Gene Code | At2g23780 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | RING/U-box superfamily protein |
| Insertion Classification | TS2TE (5') |
| Confirmation Status | unknown |
Gene hit At1g61480
| Sequence (A. th genome BLAST matches underlined) | >18-K025794-022-019-B03-8409 AACAGATTGAAGAAAGTCATCTCGTGAACTTAAAAGAGAATACATAGAAGCAAAGAAAAG AAAAAAAATCTCCTACCTTGTAAACTGAGCCAAATCCACCTTGTCCTAGTTTATTTGAGA GACTGAAATTATCAGTGGCAGTTTGAATGGTATTCATCTCAAAGAACTTTAAACCTGAGA CATCTTGTGGCTCTAGATCATTATTCCATGCCTCTTTTGAAGCAATCTTTGATATCTTCG CTGAAACTAGACTTTCAATAATAGTCAGTGTGTTACCATGCATTAGCTAGCAAATGAATA AACAAAAAACTCGAAATCAATAGAAAGATAGTAAGATCATCGGTGCGTATGGGATCCTCC CTATAGTGAGTAAACATAAGTAAGATATACTACAATTCAACTACCACTGAATCAACAACC ATATTACAGCATCAACATAACAAAAATACTAAATGT |
| GenBank Accession | CR395332 [GenBank] |
| Graphic View | 
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| Predicted Position of Insertion | Chr1:22682541 - go to primer design |
| BLAST e Value | 5e-172 |
| Hit Clone Code (BAC ID) | T1F9 |
| Hit Gene Code | At1g61480 [Araport] [TAIR] [MIPS] [SIGnAL] |
| Gene Annotation | S-locus lectin protein kinase family protein |
| Insertion Classification | CDSi |
| Confirmation Status | confirmed, show confirmation sequences |
| Primer and wt-amplicons | show primer details |
| Other FSTs Supporting this Hit | CR395332 [GenBank] |
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Last Updated on 10.06.2021 13:37 |
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