Sequences from confirmation amplicon


Confirmation sequences for line 019C03 and hit At1g22870 are shown.
Line ID 019C03
Locus At1g22870 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:8091232
Primers used for PCR 03eq - 8474, show primer details
   
Sequence Name 45-K110825-0022-019-C03C-03eq
GenBank Accession LN505562 [GenBank]
Sequencing primer gene specific primer, 03eq: 5'-AATACGCAGGGTTCAAAAACATAG-3'
Sequence (A. th genome BLAST matches underlined) 
>45-K110825-0022-019-C03C-03eq
GCATACATATTGGTTAGAGATTTACCTTGTTAATAATTAGCTCTGCACGCTTTATAAGCA
ACAGTAATGTATCACCCGTAGCAGTACTTAAGACAGGAACAAGGGCTGGGAGCGTTGTCA
ACTCAAAGTCATTTTTATCCTGCAAATCATTATTTAAGGTCTCAAGCATCATTAAAGCAT
GAGTAAAGATCTAAAAGAAATTCTTGTCCAAGATCAATGCAACTTCCAAGTAACACATGA
CAGGTTGATAATGCCGGTATTTATTTGTGCAATCACCATATGTACAACTGAAATAATCAG
GTCTAAGTATATGTGCATGTGATAGGTCATATAACACAAGTAATAAGAAAAACAAATTTT
ATATAGAATCACTGGTATCTGTAAACCTCCAATAGAATACGATCTGATTGACGAAATGTA
AGTACTTAAGATTAGAAAAAGCACCTGAGACTCTGCTATAGTTAGAACCATGGGAAGAAT
CACTGGTTGCATAACCAAATTCCTAAGTTCAGCACAAAGAGGAGGAAGCACCTGCTTGAC
AAAACCTGGTTTGATTAGTTGTTACTAGGCAAATCACTTGAGTAACTGATTTGTTTTCTG
TTAAGTAACAGAATACGCAAAAGAATCCAGAAAGTGACTACCTTATACAATTACGATATG
GCTTCATATCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAA
AAGAAAGAGTAATTACCAATTTTTTTTCAA
   
Sequence Name 29-K110824-0022-019-C03C-8409
GenBank Accession LN499340 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K110824-0022-019-C03C-8409
TCGGATATGAGCCTATCGAATTGTATAGGTAGTCACTTTCTGGATTCTTTTGCGTATTCT
GTTACTTAACAGAAAACAAATCAGTTACTCAAGTGATTTGCCTAGTAACAACTAATCAAA
CCAGGTTTTGTCAAGCAGGTGCTTCCTCCTCTTTGTGCTGAACTTAGGAATTTGGTTATG
CAACCAGTGATTCTTCCCATGGTTCTAACTATAGCAGAGTCTCAGGTGCTTTTTCTAATC
TTAAGTACTTACATTTCGTCAATCAGATCGTATTCTATTGGAGGTTTACAGATACCAGTG
ATTCTATATAAAATTTGTTTTTCTTATTACTTGTGTTATATGACCTATCACATGCACATA
TACTTAGACCTGATTATTTCAGTTGTACATATGGTGATTGCACAAATAAATACCGGCATT
ATCAACCTGTCATGTGTTACTTGGAAGTTGCATTGATCTTGGACAAGAATTTCTTTTAGA
TCTTTACTCATGCTTTAATGATGCTTGAGACCTTAAATAATGATTTGCAGGATAAAAATG
ACTTTGAGTTGACAACGCTCCCAGCCCTTGTTCCTGTCTTAAGTACTGCTACGGGTGATA
CATTACTGTTGCTTATAAAGCGTGCAGAGCTAATTATTAACAAGGTAAATCTCTAACCAA
TATGTATGCATAACTAAAAGTCTAAAACTATGTGATGTATGCATAACTGGAACTATGTTT
TTGAA

Last Updated on 06.12.2018 16:11