Sequences from confirmation amplicon


Confirmation sequences for line 019D09 and hit At4g17160 are shown.
Line ID 019D09
Locus At4g17160 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr4:9642272
Primers used for PCR 13lq - 8474, show primer details
   
Sequence Name 17-K114053-0022-019-D09C-13lq
GenBank Accession LN493937 [GenBank]
Sequencing primer gene specific primer, 13lq: 5'-CGCAAAACACATCTTCATTTTAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>17-K114053-0022-019-D09C-13lq
CATCTGTTCTGAACTCACACAACACAGTCGCTAAATTGAACTCCAAAACGAAATACATTA
CAACAAGAGAAAGAAGAAAACACATTTTTTCTATATCATATGTTTCCCATCTCAGCCGCA
GCACCCTCTTCTTTGCTGTGAGGAGGATGCATCTTTTCCACCAAACGGCCCTGGAGTTAT
TCCAGGCTCATTTGCCTCATCAACCACACCATCTTGAATCCTTTTGTATATTGTTGCGGC
TGTCTCGACGAATGCCTAGCCAAAAATTAAAAAACACGAAACACGTCGACATAATGATTT
AGACTTTCTATTAGTGTGTTAGGACTGGAGTGGAACAAGTAAGAAACTGGTGGAGATACA
AGAAGTTGGGGAACACTAGAGAAAGCATGTGTAGAGATCTCTGTAGAAACTGGTGGAGAT
ACAGGATATATTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATG
AGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAA
AATGTAGATGTCCG
   
Sequence Name 01-K114052-0022-019-D09C-8409
GenBank Accession LN486660 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K114052-0022-019-D09C-8409
ATTTCATTGAATATATCCTGTATCTCCACCAGTTTCTACAGAGATCTCTACACATGCTTT
CTCTAGTGTTCCCCAACTTCTTGTATCTCCACCAGTTTCTTACTTGTTCCACTCCAGTCC
TAACACACTAATAGAAAGTCTAAATCATTATGTCGACGTGTTTCGTGTTTTTTAATTTTT
GGCTAGGCATTCGTCGAGACAGCCGCAACAATATACAAAAGGATTCAAGATGGTGTGGTT
GATGAGGCAAATGAGCCTGGAATAACTCCAGGGCCGTTTGGTGGAAAAGATGCATCCTCC
TCACAGCAAAGAAGAGGGTGCTGCGGCTGAGATGGGAAACATATGATATAGAAAAAATGT
GTTTTCTTCTTTCTCTTGTTGTAATGTATTTCGTTTTGGAGTTCAATTTAGCGACTGTGT
TGTGGTGAGTTTCAGAACAGATTGACAATACATGTTTTTGTCTATTCACATATCTNAAAT
GAAGATTGNTTTTGCG

Last Updated on 06.12.2018 16:11