Sequences from confirmation amplicon


Confirmation sequences for line 020D01 and hit At2g40765 are shown.
Line ID 020D01
Locus At2g40765 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:17013216
Primers used for PCR CK24 - 8474, show primer details
   
Sequence Name 18-K017884-022-020-D01C-CK24
GenBank Accession LN494104 [GenBank]
Sequencing primer gene specific primer, CK24: 5'-TATGGAAAAGGTTGCAGAGAGATT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K017884-022-020-D01C-CK24
CAAAAAAAAATTTTCCCTTTGGGGCCGCCCCCGTCCTTAATGAAGGTAAGGCAAAGGAAA
ACCCCTTTAATTAATCCATTGTATTGCAACAGACATTACTGCGTTAGGTCTTCTATACTT
TGACATGGAAATAATAACTCATGATAAATGCAAAATGGGTTCTCTTTTCAAGTAACTTGG
AGTAAGTGCTTTTCTTGTTTGTTATAACATTTGAGAAGACAAATCAATTCCGAAATGGCT
TCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAG
AAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATA
AAATGAAAGTACATTTTGATAAAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAG
CAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGACGTGTCTAC
   
Sequence Name 19-K017884-022-020-D01C-8409
GenBank Accession LN494553 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>19-K017884-022-020-D01C-8409
TAGATTTCCCGGACATGAAGCCATTTCGGAATTGATTTGTCTTCTCAAATGTTATAACAA
ACAAGAAAAGCACTTACTCCAAGTTACTTGAAAAAAAAACCCATTTTGCATTTATCATGA
GTTATTATTTCCATGTCAAAGTATAAAAAACCTAACGCAGTAATGTCTGTTGCAATACAA
TGAATAAATAAAAAAGCTTATCCTTTGCCTAACCTACATAATGAACGTGGACGACACAAA
AAAAAAAGTATTATTTCAAGCAGCTTCTCTCTTCACCATCTTATGTGCATACAACAAAAC
GCTCCAACAAACATTTGGCTGGAACCAATCTCTCTGCA

Last Updated on 06.12.2018 16:11