Sequences from confirmation amplicon


Confirmation sequences for line 020D04 and hit At3g48000 are shown.
Line ID 020D04
Locus At3g48000 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:17718998
Primers used for PCR CS50 - 8474, show primer details
   
Sequence Name 26-K104847-0022-020-D04C-8409
GenBank Accession LN497916 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K104847-0022-020-D04C-8409
TTACTGAAGCCTTTACAATTGACCAGCATAGTTCGTTTTCTTTCGTTTTATGATATCGCA
ATGTTCATTGCAGTCGTTTCATTGTCCCATGTCTCTAGAGACGCGAGCTCTTCGCTGTGT
TTCTCAACCAAATCTGCAAACCTCAACAAAACTCTTGACCTTTCCTGAAACCCAAAACAA
TAACCATATGTGACCATCCATATCACATTAAAGTTCATAAAACTGAAGACTCAAGAAGAG
TAGTAATATACATACATAAGCACTCATTTTAGGCCAAGGTCCTTCATCAAAGGCCGTCCT
TGCAGCTTTCACAGCTCGATTGATATCTTCAGCATCGCCTTCAGCTACATGAGCAATGAC
TTCGCCTGTCCTCGGATCAAGAGTCGGAAACGTCTTACCTAAATCCCCAATCCCAAATA
   
Sequence Name 11-K104846-0022-020-D04C-CS50
GenBank Accession LN491102 [GenBank]
Sequencing primer gene specific primer, CS50: 5'-ATATTTGGATTTGGGGATTTAGGT-3'
Sequence (A. th genome BLAST matches underlined) 
>11-K104846-0022-020-D04C-CS50
GCGAAGTCTTGCTCAGTAGCTGAAGGCGATGCTGAAGATATCAATCGAGCTGTGAAAGCT
GCAAGGACGGCCTTTGATGAAGGACCTTGGCCTAAAATGAGTGCTTATGTATGTATATTA
CTACTCTTCTTGAGTCTTCAGTTTTATGAACTTTAATGTGATATGGATGGTCACATATGG
TTATTGTTTTGGGTTTCAGGAAAGGTCAAGAGTTTTGTTGAGGTTTGCAGATTTGGTTGA
GAAACACAGCGAAGAGCTCGCGTCTCTAGAGACATGGGACAATGAAACGACTGCAATGAA
CATTGCGATATCATAAAACGAAAGAAAACGAACTATGCTGGTCAATTGTAAATGGCTTCA
TGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGATGGTCAATA
TGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAGATGTCCGCA

Last Updated on 06.12.2018 16:11