Sequences from confirmation amplicon


Confirmation sequences for line 020E05 and hit At1g21695 are shown.
Line ID 020E05
Locus At1g21695 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:7618962
Primers used for PCR UF20 - 8474, show primer details
   
Sequence Name 01-K030138-022-020-E05C-8409
GenBank Accession LN486448 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K030138-022-020-E05C-8409
AATTGGATATATCCTTGACAGCATTTTTATTTATTTATGGCATTACTAATGTTTTCTATA
GTTTGGATGTGTATCCGAATCTTCCTTTTATGTGGATATGTATATTAATCCTCTTGTATT
TTATTACATTTTCGTAGGATTTTACTATTTTGATTATTAGAGGTAATAAATAGAAAAATA
TAATTATATAAATCTACTATATTTTTTATTTTTATATTTACATTTTGGGATCTACACTGT
ATATATATCCAACTAGTAAAGAAACACATTACTCCTTTTACGGTGTCAATATTGGATATA
GATAAAGGGATTCACTAATTCTTACAACAGAAGCAATGTCCATCAAGCTATCTGGTCATG
ATTTT
   
Sequence Name 01-K030138-022-020-E05C-UF20
GenBank Accession LN486449 [GenBank]
Sequencing primer gene specific primer, UF20: 5'-CAAAATCATGACCAGATAGCTTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>01-K030138-022-020-E05C-UF20
TCCCTTTATCTATATCCAATATTGACACCGTAAAAGGAGTAATGTGTTTCTTTACTAGTT
GGATATATATACAGTGTAGATCCCAAAATGTAAATATAAAAATAAAAAATATAGTAGATT
TATATAATTATATTTTTCTATTTATTACCTCTAATAATCAAAATAGTAAAATCCTACGAA
AATGTAATAAAATACAAGAGGATTAATATACATATCCACATAAAAGGAAGATTCGGATAC
ACATCCAAACTATAGAAAACATTAGTAATGCCATAAATAAATAAAAATGCTGTCAAGGAT
ATATCCAATTGTAAATGGCTTCATGTCCGGGAAATCTACATGGATCAGCAATGAGTATGA
TGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTTTTCAATTCAAAAATGTAG
ATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATAAAACGACAAATTACGATCC
GTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAATTCGGAAATCTTTATTTCGA
CGTGTCTACATTCACGTCCAAATGGGGAGGG
Primers used for PCR UF21 - 8474, show primer details
   
Sequence Name 09-K030139-022-020-E05C-8409
GenBank Accession LN490051 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K030139-022-020-E05C-8409
AGCCATTTACAATTGAAGACACAAAATTCGTTGAATGTTATAGTCTTCACATAAAACTGT
CATAAATCTGGATACGTTCTAAAAATAATTGATCTAATCTAAATCTATAAGTAGTTTCTT
ATTAGTAGAATCCTTATGAATCTTAATTTGATCAAGACAGAAACGCTTTTGTTTACTTTT
ACCTAATGGTCGATGGTGACGATTCTTTCAAGAGACCCGGAGCTGTGCCTTTTAAGTGGG
AGATCCGACCCGGTGTACCTAAAACCCGACCCGGTGATGATGATCCAGCTCTTCTTCAAC
CACCTCCTCCTAACAAACTTCCTCCTCTCAAATTAAAATCAACTCCGCCGTCGAATTCGC
CATCTCCGTCGTCTTCTTCTTCTTTCTTCTCTGAATCCAGAA
   
Sequence Name 09-K030139-022-020-E05C-UF21
GenBank Accession LN490052 [GenBank]
Sequencing primer gene specific primer, UF21: 5'-GCTTCTGGATTCAGAGAAGAAAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>09-K030139-022-020-E05C-UF21
ATTTTAATTTGAGAGGAGGAAGTTTGTTAGGAGGAGGTGGTTGAAGAAGAGCTGGATCAT
CATCACCGGGTCGGGTTTTAGGTACACCGGGTCGGATCTCCCACTTAAAAGGCACAGCTC
CGGGTCTCTTGAAAGAATCGTCACCATCGACCATTAGGTAAAAGTAAACAAAAGCGTTTC
TGTCTTGATCAAATTAAGATTCATAAGGATTCTACTAATAAGAAACTACTTATAGATTTA
GATTAGATCAATTATTTTTAGAACGTATCCAGATTTATGACAGTTTTATGTGAAGACTAT
AACATTCAACGAATTTTGTGTCTTCAATTGTAAATGGCTTCATGTCCGGGAAATCTACAT
GGATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTT
TTTCAATTCAAAAATGTAGATGTCCGCAGCGTTATTATAAAATGAAAGTACATTTTGATA
AAACGACAAATTACGATCCGTCGTATTTATAGGCGAAAGCAATAAACAAATTATTCTAAT
TCGGAAATCTTTATTTCGACGTGTCTACATTCACG

Last Updated on 06.12.2018 16:11