Sequences from confirmation amplicon


Confirmation sequences for line 020E05 and hit At5g16400 are shown.
Line ID 020E05
Locus At5g16400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr5:5364112
Primers used for PCR PG04 - 8474, show primer details
   
Sequence Name 39-K016588-022-020-E05C-8409
GenBank Accession LN503175 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>39-K016588-022-020-E05C-8409
GTAGATTTCCCGGACAAACCTTGTTGTCTTGGTTACAATCAAGCTTAAGAAACACCATGT
CCTGGTACTTCTCCGATAGCTCTTTGTATTTGGGAGCAATCACTTTGCAAGGACCACACC
TGCAAAGAGCCAAACGAGGATTGAAAAAAAAGAGAGGCAAACTGATTTTTCTCAACACCA
AAACAGAACATTTAATAGTAAACTCATCTCAAGCATTCATGATGATGGAAATTGGAACAC
CTTTTATGCTTATTTATTAAATGTACATGCTCTAATTCTCCTGAAAGTCTCACTATTCAT
AACTTCATATCCTTTTGTATATATCATTCCTAACCAAAAAATGAATTCAAAAGAATGTGT
ACAGAGAAGCAGCAGCCTGATACTGTGAAGATTATATATGATACTTACAATGCTGTAACT
TTTGAATACAATTGAAAGTAACAAGGTGATCATATACTAACCCTAAACTATGGAAGCTAC
ACAATGTGAGGTTCATAGAGCTAAAAATAGGCCAAGAGAAGCAAAGGATATGGTTAGAGA
CCATGATATGATTATGTTGTAGCAAGAATTGGCAATTGAGAATTAAAGACATGAGGATCT
GCTAAAGCAAAGTTCTCTGGTAAACAACTTAGCAATGTTATATCAACTGAGAAATCCCTA
ATCAACCCTAATTTC
   
Sequence Name 43-K016588-022-020-E05C-PG04
GenBank Accession LN504661 [GenBank]
Sequencing primer gene specific primer, PG04: 5'-TTGGATAAGTCGTTGGAAATTAGG-3'
Sequence (A. th genome BLAST matches underlined) 
>43-K016588-022-020-E05C-pg04
GGATTTCTCAGTTGATATAACATTGCTAAGTTGTTTACCAGAGAACTTTGCTTTAGCAGA
TCCTCATGTCTTTAATTCTCAATTGCCAATTCTTGCTACAACATAATCATATCATGGTCT
CTAACCATATCCTTTGCTTCTCTTGGCCTATTTTTAGCTCTATGAACCTCACATTGTGTA
GCTTCCATAGTTTAGGGTTAGTATATGATCACCTTGTTACTTTCAATTGTATTCAAAAGT
TACAGCATTGTAAGTATCATATATAATCTTCACAGTATCAGGCTGCTGCTTCTCTGTACA
CATTCTTTTGAATTCATTTTTTGGTTAGGAATGATATATACAAAAGGATATGAAGTTATG
AATAGTGAGACTTTCAGGAGAATTAGAGCATGTACATTTAATAAATAAGCATAAAAGGTG
TTCCAATTTCCATCATCATGAATGCTTGAGATGAGTTTACTATTAAATGTTCTGTTTTGG
TGTTGAGAAAAATCAGTTTGCCTCTCTTTTTTTTCAATCCTCGTTTGGCTCTTTGCAGGT
GTGGTCCTTGCAAAGTGATTGCTCCCAAATACAAAGAGCTATCGGAGAAGTACCAGGACA
TGGTGTTTCTTAAGCTTGATTGTAACCAAGACAACAAGGTTTGTCCGGGAAATCTACATG
GATCAGCAATGAGTATGATGGTCAATATGGAGAAAAAGAAAGAGTAATTACCAATTTTTT
TTCAATTCAAAAA

Last Updated on 06.12.2018 16:11