Sequences from confirmation amplicon


Confirmation sequences for line 022A11 and hit At1g54270 are shown.
Line ID 022A11
Locus At1g54270 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:20260812
Primers used for PCR JA35 - 8474, show primer details
   
Sequence Name 25-K100938-0022-022-A11C-8409
GenBank Accession LN497363 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K100938-0022-022-A11C-8409
CATTTACATTGAATCCCACTAACAAACAAAGGTTGTTTCTTTAAACCTTCTTCACTTCCG
TAAGCATAGATACCCCTAAGAAGATTCTCTTGCAACCCATGGCATCAAAGCTCTCATGGA
CCTCATCATATGAGGTGAAAACTATCCTGTCCATCGAGACTGCCCCAACAAACATAGATA
GCAATACAAAAAAATCTCATCAAAACGAGTATGAACTAAG
   
Sequence Name 25-K100944-0022-022-A11C-JA35
GenBank Accession LN497364 [GenBank]
Sequencing primer gene specific primer, JA35: 5'-AGCTTTTATCTTCGATTTTGGACA-3'
Sequence (A. th genome BLAST matches underlined) 
>25-K100944-0022-022-A11C-JA35
GAGATAGATGTTTTTGATGGTATCTGTTATGATAATTGGATGAACCATATTTCCTATATC
TCTCCTGACATCGAATAATTCAGATATTAATGCTTGTGTTCCTCTCTATATGGAACTTCA
AGTTTGGCTATTCTGTTACATTCTTACTTTTGCACAACTCTCTCTGGCTATAAGATTTAA
GAATCATCTCTAGCTATCTGGGTTTCTAACTATTGTTTCTTTTGTGTA
Primers used for PCR JA36 - 8474, show primer details
   
Sequence Name 26-K100938-0022-022-A11C-OriL
GenBank Accession LN497843 [GenBank]
Sequencing primer T-DNA primer, 8409: 5'-ATATTGACCATCATACTCATTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100938-0022-022-A11C-OriL
CTTTACGTATGTGCTCTTTCCTGTGATTGATTGGTTGATTTTTTTCTAGGTTTCGAAAAG
CCTTCTGCTATTCAGCAAAGAGGAATTGTACCCTTTTGCAAGGGTCTTGATGTGATCCAG
CAGGCACAGTCTGGTACTGGAAAAACCGCCACTTTCTGCTCTGGTGTCTTGCAGCAGCTT
GACTATGCCCTTCTCCAGTGCCAGGCTCTCGTTTTGGCTCCCACCAGAGAGCTTGCTCAG
CAGATTGAGAAAGTCATGCGTGCC
   
Sequence Name 26-K100944-0022-022-A11C-JA36
GenBank Accession LN497844 [GenBank]
Sequencing primer gene specific primer, JA36: 5'-CATAGATCTGCAAATTCACCAGAA-3'
Sequence (A. th genome BLAST matches underlined) 
>26-K100944-0022-022-A11C-JA36
CAGTCATATCAGAGATAACCACGAGGGTAGATTCAAACTTTGAAGAAGATAACAAACCTG
ATCCTTGAAACCACGGGAGAGCATTTCATCAGCTTCATCAAGGACAAACATCTTGATGCA
GTCAGGGCGGAGAGATTGTCTTCTAAGCATGTCAAAAACACGACCGGGAGTTCCAACGAC
AACATGAACACCAGCCTGGAGAATGCGCTGATCCTCACGGACACTGGTTCCACCCACACA
GGCATGAACCTTGACA

Last Updated on 06.12.2018 16:11